Induced luteolysis in the primate: Rapid loss of luteinizing hormone receptors

W. C. Duncan, P. J. Illingworth, F. M. Young, H. M. Fraser

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The molecular mechanisms involved in luteolysis are still unclear in the primate. This study aimed to investigate the effect of induced luteolysis on the ovarian luteinizing hormone (LH) receptor and the steroidogenic enzyme, 3β-hydroxysteroid dehydrogenase (3β-HSD) in the marmoset monkey. Luteolysis was induced in the mid-luteal phase either directly by systemic prostaglandin F(2α) (PGF(2α)), or indirectly by LH withdrawal using systemic gonadotrophin releasing hormone antagonist (GnRH(ant)) treatment. The LH receptor was studied by isotopic mRNA in-situ hybridization and in-situ ligand binding and 3β-HSD expression was studied using isotopic mRNA in-situ hybridization and immunohistochemistry. Induced luteolysis was associated with a reduction in the expression of LH receptor (P < 0.0001) and 3β-HSD mRNA, closely followed by a reduction in the LH receptor (P < 0.05) and 3β-HSD protein concentrations within 24 h. There were no differences in the findings whether luteolysis was induced with PGF(2α) or GnRH(ant). This study shows that disparate mechanisms to induce luteolysis in the primate result in an identical rapid loss of the LH receptor and 3β-HSD. In conclusion, induced luteolysis leads to rapid loss of the steroidogenic pathway in luteal cells.
Original languageEnglish
Pages (from-to)2532-2540
Number of pages9
JournalHuman Reproduction
Volume13
Issue number9
DOIs
Publication statusPublished - Sep 1998
Externally publishedYes

Keywords

  • 3β-hydroxysteroid dehydrogenase
  • Corpus luteum
  • Immunohistochemistry
  • In-situ hybridization
  • Luteinizing hormone receptor

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