Induction of HIV-1 subtype B and AE-specific neutralizing antibodies in mice and macaques with DNA prime and recombinant gp140 protein boost regimens

Rob J. Center; Adam K. Wheatley; Shahan M. Campbell; Adriana J. Gaeguta; Viv Peut; Sheilajen Alcantara; Carly Siebentritt; Stephen J. Kent; Damian F.J. Purcell

doi:10.1016/j.vaccine.2009.08.016

Induction of HIV-1 subtype B and AE-specific neutralizing antibodies in mice and macaques with DNA prime and recombinant gp140 protein boost regimens

Rob J. Center, Adam K. Wheatley, Shahan M. Campbell, Adriana J. Gaeguta, Viv Peut, Sheilajen Alcantara, Carly Siebentritt, Stephen J. Kent, Damian F.J. Purcell

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

We developed highly expressing clade B and AE DNA and envelope protein (Env) vaccines for evaluation in mice and macaques as DNA prime/protein boost regimens. High levels of Env-specific antibodies were induced in mice, albeit with limited neutralizing activity in vitro. A combined clade B and AE regimen induced high titer Env-specific antibody in two pigtail macaques that neutralized several strains of HIV-1. However, upon mucosal challenge with SHIV_SF162P3 no protection from infection was observed. Although the vaccines tested provide a platform for inducing robust humoral immunity, further refinements to broaden coverage against divergent strains and induce mucosal immunity are needed.

Original language	English
Pages (from-to)	6605-6612
Number of pages	8
Journal	Vaccine
Volume	27
Issue number	47
DOIs	https://doi.org/10.1016/j.vaccine.2009.08.016
Publication status	Published - 5 Nov 2009
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Envelope protein
HIV-1
Vaccine

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10.1016/j.vaccine.2009.08.016Licence: In Copyright

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Center, R. J., Wheatley, A. K., Campbell, S. M., Gaeguta, A. J., Peut, V., Alcantara, S., Siebentritt, C., Kent, S. J., & Purcell, D. F. J. (2009). Induction of HIV-1 subtype B and AE-specific neutralizing antibodies in mice and macaques with DNA prime and recombinant gp140 protein boost regimens. Vaccine, 27(47), 6605-6612. https://doi.org/10.1016/j.vaccine.2009.08.016

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title = "Induction of HIV-1 subtype B and AE-specific neutralizing antibodies in mice and macaques with DNA prime and recombinant gp140 protein boost regimens",

abstract = "We developed highly expressing clade B and AE DNA and envelope protein (Env) vaccines for evaluation in mice and macaques as DNA prime/protein boost regimens. High levels of Env-specific antibodies were induced in mice, albeit with limited neutralizing activity in vitro. A combined clade B and AE regimen induced high titer Env-specific antibody in two pigtail macaques that neutralized several strains of HIV-1. However, upon mucosal challenge with SHIVSF162P3 no protection from infection was observed. Although the vaccines tested provide a platform for inducing robust humoral immunity, further refinements to broaden coverage against divergent strains and induce mucosal immunity are needed.",

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AU - Center, Rob J.

AU - Wheatley, Adam K.

AU - Campbell, Shahan M.

AU - Gaeguta, Adriana J.

AU - Peut, Viv

AU - Alcantara, Sheilajen

AU - Siebentritt, Carly

AU - Kent, Stephen J.

AU - Purcell, Damian F.J.

PY - 2009/11/5

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AB - We developed highly expressing clade B and AE DNA and envelope protein (Env) vaccines for evaluation in mice and macaques as DNA prime/protein boost regimens. High levels of Env-specific antibodies were induced in mice, albeit with limited neutralizing activity in vitro. A combined clade B and AE regimen induced high titer Env-specific antibody in two pigtail macaques that neutralized several strains of HIV-1. However, upon mucosal challenge with SHIVSF162P3 no protection from infection was observed. Although the vaccines tested provide a platform for inducing robust humoral immunity, further refinements to broaden coverage against divergent strains and induce mucosal immunity are needed.

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Induction of HIV-1 subtype B and AE-specific neutralizing antibodies in mice and macaques with DNA prime and recombinant gp140 protein boost regimens

Abstract

UN SDGs

Keywords

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