Influence of immobilized quaternary ammonium group surface density on antimicrobial efficacy and cytotoxicity

Alex Cavallaro, Agnieszka Mierczynska, Mary Barton, Peter Majewski, Krasimir Vasilev

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Bacterial colonization of medical devices causes infections and is a significant problem in healthcare. The use of antibacterial coatings is considered as a potential solution to this problem and has attracted a great deal of attention. Using concentration density gradients of immobilized quaternary ammonium compounds it was demonstrated that a specific threshold of surface concentration is required to induce significant bacterial death. It was determined that this threshold was 4.18% NR4+ bonded nitrogen with a surface potential of + 120.4 mV. Furthermore, it is shown for the first time that adhesion of constituents of the culture medium to the quaternary ammonium modified surface eliminated any cytotoxicity towards eukaryotic cells such as primary human fibroblasts. The implications of this type of surface fouling on the antimicrobial efficacy of surface coatings are also discussed.

Original languageEnglish
Pages (from-to)13-24
Number of pages12
Journalbiofouling
Volume32
Issue number1
DOIs
Publication statusPublished - 2016
Externally publishedYes

Keywords

  • antibacterial
  • biological fouling
  • cytotoxicity
  • Density gradients
  • quaternary ammonium

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