In this study, insulin-like growth factor I (IGF I) and IGF II in golden pompano larvae were cloned and analyzed. In the first trial, IGF expression during ontogeny of larvae in the first 18-days of their life was explored, and then the response of IGFs to water temperature (23, 26, and 29°C) on 12 day post hatching (DPH) and 18 DPH were compared. On 28 DPH, the response of IGFs to the manipulation of nutrients was evaluated. The expression of IGF I increased with the increase of fish age, and was not significantly affected by water temperature. The expression of IGF II was affected by water temperature on 12 DPH and 18 DPH. The expression of IGF II at 23°C was significantly higher than at 26°C and 29°C. The expression of IGFs in fish larvae on 28 DPH was not concomitant with nutrient manipulation. This study detected the gene expression of IGFs at the early stage of golden pompano larvae. The time dependent expression of IGF genes in fish larvae is important to understand the ontogenetic development and growth of fish larvae in early life.
|Number of pages||14|
|Journal||Israeli Journal of Aquaculture-Bamidgeh|
|Issue number||article ID 1336|
|Publication status||Published - 2016|
- Gene expression
- Golden pompano Trachinotus ovatus
- Insulin-like growth factors