Interferometric porous silicon transducers using an enzymatically amplified optical signal

Endre Szili, Andrew Jane, Suet Low, Martin Sweetman, Peter MacArdle, Sunil Kumar, Roger Smart, Nicolas Voelcker

    Research output: Contribution to journalArticlepeer-review

    40 Citations (Scopus)

    Abstract

    This paper describes a novel transducer principle converting biomolecular binding events into changes in porous silicon structural colour. An enzyme-catalysed reaction, horseradish peroxidase (HRP) mediated oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB), induced an amplified optical detection signal on an antibody-functionalised porous silicon film, which manifested as significant changes in the Fabry-Pérot fringe pattern. Exposing porous silicon to non-oxidised TMB and oxidised TMB products and measuring the resulting effective optical thickness (EOT) changes of the porous silicon layer, an amplified signal from porous silicon was found, caused by an intermediate radical cation produced during the enzymatic-catalysed oxidation of TMB. Finally, it was shown that this system could be used to detect a human immunoglobulin (IgG) at 0.2 μg/ml. This work may lead to the development of new biosensors where porous silicon acts as the sensing matrix and transducer element.

    Original languageEnglish
    Pages (from-to)341-348
    Number of pages8
    JournalSensors and Actuators B: Chemical
    Volume160
    Issue number1
    DOIs
    Publication statusPublished - 15 Dec 2011

    Keywords

    • 3,3′,5,5′-Tetramethylbenzidine
    • Horseradish peroxidase
    • Human immunoglobulin
    • Interferometric reflectance spectroscopy
    • Porous silicon

    Fingerprint Dive into the research topics of 'Interferometric porous silicon transducers using an enzymatically amplified optical signal'. Together they form a unique fingerprint.

    Cite this