Interindividual variation in mitotic recombination

Deborah Holt, Monica Dreimanis, Marie Pfeiffer, Frank Firgaira, Alec Morley, David Turner

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    26 Citations (Scopus)

    Abstract

    Mitotic recombination (MR) between homologous chromosomes is a mutational event that results in loss of heterozygosity in half of the segregants at mitosis. Loss of heterozygosity may have important biological consequences. The purpose of this study was to describe human variation in the spontaneous frequency of MR. Using an immunoselection technique for isolating the somatic mutations that result in loss of expression of one of the codominant alleles at the HLA-A locus, we have measured the frequency and molecular basis of somatic mutations in lymphocytes from a population of young adults. Mutations were classified as being the result of intragenic changes, major deletions, or MR. Here we show that the MR mutation frequency in females was significantly greater than that in males but that intragenic mutation frequency showed no association with sex. Individual variation in MR frequency ranged over more than two orders of magnitude and was not normally distributed. Furthermore, the observed number of individuals from whom no mutants resulting from MR were obtained was significantly greater than was expected. The endogenous level of MR may be under genetic control. Given the association of loss of heterozygosity with cancer initiation and progression, low endogenous MR may confer a reduced lifetime risk of cancer, and the converse may apply.

    Original languageEnglish
    Pages (from-to)1423-1427
    Number of pages5
    JournalAmerican Journal of Human Genetics
    Volume65
    Issue number5
    DOIs
    Publication statusPublished - Nov 1999

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