Intermittent ischaemia maintains function after ischaemia reperfusion in steatotic livers

M Steenks, Mark Van Baal, Vincent Nieuwenhuijs, Menno De Bruijn, Marc Schiesser, E Teo, Tom Callahan, Robert Padbury, Gregory Barritt

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    9 Citations (Scopus)

    Abstract

    Background: Ischaemic preconditioning (IPC) and intermittent ischaemia (INT) reduce liver injury after ischaemia reperfusion (IR). Steatotic livers are at a higher risk of IR injury, but the protection offered by IPC and INT is not well understood. The aim of the present study was to determine the effectiveness of IPC and INT in maintaining liver function in steatotic livers. Material and methods: A model of segmental hepatic ischaemia (45 min) and reperfusion (60 min) was employed using lean and obese Zucker rats. Bile flow recovery was measured to assess dynamic liver function, hepatocyte fat content quantified and blood electrolytes, metabolites and bile calcium measured to assess liver and whole body physiology. Liver marker enzymes and light and electron microscopy were employed to assess hepatocyte injury. Results: IPC was not effective in promoting bile flow recovery after IR in either lean or steatotic livers, whereas INT promoted good bile flow recovery in steatotic as well as lean livers. However, the bile flow recovery in steatotic livers was less than that in lean livers. In steatotic livers, ischaemia led to a rapid and substantial decrease in fat content. Steatotic livers were more susceptible to IR injury than lean livers, as indicated by increased blood ALT concentrations and major histological injury. Conclusion: INT is more effective than IPC in restoring liver function in the acute phase of IR in steatotic livers. In obese patients, INT may be useful in promoting better liver function after IR after liver resection.

    Original languageEnglish
    Article numberPublications Data Collection
    Pages (from-to)250-261
    Number of pages12
    JournalJournal of Hepato-Biliary-Pancreatic Surgery
    Volume12
    Issue number4
    DOIs
    Publication statusPublished - May 2010

    Keywords

    • Bile flow
    • Intermittent ischaemia
    • Ischaemia reperfusion injury
    • Ischaemic preconditioning
    • Lipid
    • Liver
    • Obese
    • Rat

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    Steenks, M., Van Baal, M., Nieuwenhuijs, V., De Bruijn, M., Schiesser, M., Teo, E., Callahan, T., Padbury, R., & Barritt, G. (2010). Intermittent ischaemia maintains function after ischaemia reperfusion in steatotic livers. Journal of Hepato-Biliary-Pancreatic Surgery, 12(4), 250-261. [Publications Data Collection]. https://doi.org/10.1111/j.1477-2574.2010.00160.x