Internal validation of STRmix™: A multi laboratory response to PCAST

Jo Anne Bright; Rebecca Richards; Maarten Kruijver; Hannah Kelly; Catherine McGovern; Alan Magee; Andrew McWhorter; Anne Ciecko; Brian Peck; Chase Baumgartner; Christina Buettner; Scott McWilliams; Claire McKenna; Colin Gallacher; Ben Mallinder; Darren Wright; Deven Johnson; Dorothy Catella; Eugene Lien; Craig O'Connor; George Duncan; Jason Bundy; Jillian Echard; John Lowe; Joshua Stewart; Kathleen Corrado; Sheila Gentile; Marla Kaplan; Michelle Hassler; Naomi McDonald; Paul Hulme; Rachel H. Oefelein; Shawn Montpetit; Melissa Strong; Sarah Noël; Simon Malsom; Steven Myers; Susan Welti; Tamyra Moretti; Teresa McMahon; Thomas Grill; Tim Kalafut; Mary Margaret Greer-Ritzheimer; Vickie Beamer; Duncan A. Taylor; John S. Buckleton

doi:10.1016/j.fsigen.2018.01.003

Internal validation of STRmix™: A multi laboratory response to PCAST

Jo Anne Bright, Rebecca Richards, Maarten Kruijver, Hannah Kelly, Catherine McGovern, Alan Magee, Andrew McWhorter, Anne Ciecko, Brian Peck, Chase Baumgartner, Christina Buettner, Scott McWilliams, Claire McKenna, Colin Gallacher, Ben Mallinder, Darren Wright, Deven Johnson, Dorothy Catella, Eugene Lien, Craig O'ConnorGeorge Duncan, Jason Bundy, Jillian Echard, John Lowe, Joshua Stewart, Kathleen Corrado, Sheila Gentile, Marla Kaplan, Michelle Hassler, Naomi McDonald, Paul Hulme, Rachel H. Oefelein, Shawn Montpetit, Melissa Strong, Sarah Noël, Simon Malsom, Steven Myers, Susan Welti, Tamyra Moretti, Teresa McMahon, Thomas Grill, Tim Kalafut, Mary Margaret Greer-Ritzheimer, Vickie Beamer, Duncan A. Taylor, John S. Buckleton

College of Science and Engineering

Research output: Contribution to journal › Article › peer-review

77 Citations (Scopus)

Abstract

We report a large compilation of the internal validations of the probabilistic genotyping software STRmix™. Thirty one laboratories contributed data resulting in 2825 mixtures comprising three to six donors and a wide range of multiplex, equipment, mixture proportions and templates. Previously reported trends in the LR were confirmed including less discriminatory LRs occurring both for donors and non-donors at low template (for the donor in question) and at high contributor number. We were unable to isolate an effect of allelic sharing. Any apparent effect appears to be largely confounded with increased contributor number.

Original language	English
Pages (from-to)	11-24
Number of pages	14
Journal	Forensic Science International: Genetics
Volume	34
DOIs	https://doi.org/10.1016/j.fsigen.2018.01.003
Publication status	Published - May 2018

Keywords

Continuous models
Forensic DNA
PCAST
Probabilistic genotyping
STRmix
Validation

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10.1016/j.fsigen.2018.01.003

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Bright, J. A., Richards, R., Kruijver, M., Kelly, H., McGovern, C., Magee, A., McWhorter, A., Ciecko, A., Peck, B., Baumgartner, C., Buettner, C., McWilliams, S., McKenna, C., Gallacher, C., Mallinder, B., Wright, D., Johnson, D., Catella, D., Lien, E., ... Buckleton, J. S. (2018). Internal validation of STRmix™: A multi laboratory response to PCAST. Forensic Science International: Genetics, 34, 11-24. https://doi.org/10.1016/j.fsigen.2018.01.003

@article{d25f90eb15244fddb6dca3a4a9f42f26,

title = "Internal validation of STRmix{\texttrademark}: A multi laboratory response to PCAST",

abstract = "We report a large compilation of the internal validations of the probabilistic genotyping software STRmix{\texttrademark}. Thirty one laboratories contributed data resulting in 2825 mixtures comprising three to six donors and a wide range of multiplex, equipment, mixture proportions and templates. Previously reported trends in the LR were confirmed including less discriminatory LRs occurring both for donors and non-donors at low template (for the donor in question) and at high contributor number. We were unable to isolate an effect of allelic sharing. Any apparent effect appears to be largely confounded with increased contributor number.",

keywords = "Continuous models, Forensic DNA, PCAST, Probabilistic genotyping, STRmix, Validation",

author = "Bright, {Jo Anne} and Rebecca Richards and Maarten Kruijver and Hannah Kelly and Catherine McGovern and Alan Magee and Andrew McWhorter and Anne Ciecko and Brian Peck and Chase Baumgartner and Christina Buettner and Scott McWilliams and Claire McKenna and Colin Gallacher and Ben Mallinder and Darren Wright and Deven Johnson and Dorothy Catella and Eugene Lien and Craig O'Connor and George Duncan and Jason Bundy and Jillian Echard and John Lowe and Joshua Stewart and Kathleen Corrado and Sheila Gentile and Marla Kaplan and Michelle Hassler and Naomi McDonald and Paul Hulme and Oefelein, {Rachel H.} and Shawn Montpetit and Melissa Strong and Sarah No{\"e}l and Simon Malsom and Steven Myers and Susan Welti and Tamyra Moretti and Teresa McMahon and Thomas Grill and Tim Kalafut and Greer-Ritzheimer, {Mary Margaret} and Vickie Beamer and Taylor, {Duncan A.} and Buckleton, {John S.}",

year = "2018",

month = may,

doi = "10.1016/j.fsigen.2018.01.003",

language = "English",

volume = "34",

pages = "11--24",

journal = "Forensic Science International: Genetics",

issn = "1872-4973",

publisher = "Elsevier B.V.",

}

Bright, JA, Richards, R, Kruijver, M, Kelly, H, McGovern, C, Magee, A, McWhorter, A, Ciecko, A, Peck, B, Baumgartner, C, Buettner, C, McWilliams, S, McKenna, C, Gallacher, C, Mallinder, B, Wright, D, Johnson, D, Catella, D, Lien, E, O'Connor, C, Duncan, G, Bundy, J, Echard, J, Lowe, J, Stewart, J, Corrado, K, Gentile, S, Kaplan, M, Hassler, M, McDonald, N, Hulme, P, Oefelein, RH, Montpetit, S, Strong, M, Noël, S, Malsom, S, Myers, S, Welti, S, Moretti, T, McMahon, T, Grill, T, Kalafut, T, Greer-Ritzheimer, MM, Beamer, V, Taylor, DA & Buckleton, JS 2018, 'Internal validation of STRmix™: A multi laboratory response to PCAST', Forensic Science International: Genetics, vol. 34, pp. 11-24. https://doi.org/10.1016/j.fsigen.2018.01.003

TY - JOUR

T1 - Internal validation of STRmix™

T2 - A multi laboratory response to PCAST

AU - Bright, Jo Anne

AU - Richards, Rebecca

AU - Kruijver, Maarten

AU - Kelly, Hannah

AU - McGovern, Catherine

AU - Magee, Alan

AU - McWhorter, Andrew

AU - Ciecko, Anne

AU - Peck, Brian

AU - Baumgartner, Chase

AU - Buettner, Christina

AU - McWilliams, Scott

AU - McKenna, Claire

AU - Gallacher, Colin

AU - Mallinder, Ben

AU - Wright, Darren

AU - Johnson, Deven

AU - Catella, Dorothy

AU - Lien, Eugene

AU - O'Connor, Craig

AU - Duncan, George

AU - Bundy, Jason

AU - Echard, Jillian

AU - Lowe, John

AU - Stewart, Joshua

AU - Corrado, Kathleen

AU - Gentile, Sheila

AU - Kaplan, Marla

AU - Hassler, Michelle

AU - McDonald, Naomi

AU - Hulme, Paul

AU - Oefelein, Rachel H.

AU - Montpetit, Shawn

AU - Strong, Melissa

AU - Noël, Sarah

AU - Malsom, Simon

AU - Myers, Steven

AU - Welti, Susan

AU - Moretti, Tamyra

AU - McMahon, Teresa

AU - Grill, Thomas

AU - Kalafut, Tim

AU - Greer-Ritzheimer, Mary Margaret

AU - Beamer, Vickie

AU - Taylor, Duncan A.

AU - Buckleton, John S.

PY - 2018/5

Y1 - 2018/5

N2 - We report a large compilation of the internal validations of the probabilistic genotyping software STRmix™. Thirty one laboratories contributed data resulting in 2825 mixtures comprising three to six donors and a wide range of multiplex, equipment, mixture proportions and templates. Previously reported trends in the LR were confirmed including less discriminatory LRs occurring both for donors and non-donors at low template (for the donor in question) and at high contributor number. We were unable to isolate an effect of allelic sharing. Any apparent effect appears to be largely confounded with increased contributor number.

AB - We report a large compilation of the internal validations of the probabilistic genotyping software STRmix™. Thirty one laboratories contributed data resulting in 2825 mixtures comprising three to six donors and a wide range of multiplex, equipment, mixture proportions and templates. Previously reported trends in the LR were confirmed including less discriminatory LRs occurring both for donors and non-donors at low template (for the donor in question) and at high contributor number. We were unable to isolate an effect of allelic sharing. Any apparent effect appears to be largely confounded with increased contributor number.

KW - Continuous models

KW - Forensic DNA

KW - PCAST

KW - Probabilistic genotyping

KW - STRmix

KW - Validation

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U2 - 10.1016/j.fsigen.2018.01.003

DO - 10.1016/j.fsigen.2018.01.003

M3 - Article

C2 - 29367014

AN - SCOPUS:85041379027

SN - 1872-4973

VL - 34

SP - 11

EP - 24

JO - Forensic Science International: Genetics

JF - Forensic Science International: Genetics

ER -

Internal validation of STRmix™: A multi laboratory response to PCAST

Abstract

Keywords

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