Intrahypothalamic actions of somatostatin and growth hormone releasing factor on growth hormone secretion

Possible effects of GRF on somatostatin neurons and of somatostatin on GRF neurons were examined by measuring the effects oflocalised intracerebral injections of these peptides on growth hormone (GH) secretion. Serial GH concentrations were measured in plasma in unanaesthetized male rats chronically prepared with venous and intracerebral cannuiae, before and after treatment with bilateral intracerebral injections of somatostatin or GRF in the preoptic anterior hypothalamic area (PO/AHA) and medial basal hypothalamus. Injections of 0.1 and Inmol of GRF in medial basal hypothalamus or 10 nmoi somatostatin in the PO/AHA, respectively, had stimulatory or inhibitory effects on GH. which were assumed to be due to diffusion of the peptide from the injection site to the median eminence and pituitary giand. Injection of lower doses of somatostatin around GRF neurons in the medial basal hypothalamus were without significant effect on secretion ofGH. but U. I nmol somatostatin in the PO/AHA resulted in an increase in GH concentrations from 128 ± 61 to 524 ± 103 ng/ml, p<0.02. Injections of GRF in lower doses amongst somatostatin neurons in the PO/AH or amongst GRF neurons in the medial basal hypothalamus were both without effect on GH secretion. We conclude that somatostatin may stimulate GH secretion by an effect on or close to somatostatin neurons in the PO/AHA. Somatostatin, though present in terminals on GRF neurons, is without effect at Ihis site in our model. Furthermore, we have been unable to demonstrate any sigificant intrahypothalamic effect of GRF on GH regulation.