Inulin isoforms differ by repeated additions of one crystal unit cell

Peter Cooper, Thomas Barclay, Milena Ginic-Markovic, Andrea Gerson, Nikolai Petrovsky

    Research output: Contribution to journalArticlepeer-review

    20 Citations (Scopus)

    Abstract

    Inulin isoforms, especially delta inulin, are important biologically as immune activators and clinically as vaccine adjuvants. In exploring action mechanisms, we previously found regular increments in thermal properties of the seven-member inulin isoform series that suggested regular additions of some energetic structural unit. Because the previous isolates carried additional longer chains that masked defining ranges, these were contrasted with new isoform isolates comprising only inulin chain lengths defining that isoform. The new series began with 19 fructose units per chain (alpha-1 inulin), increasing regularly by 6 fructose units per isoform. Thus the 'energetic unit' equates to 6 fructose residues per chain. All isoforms showed indistinguishable X-ray diffraction patterns that were also identical with known inulin crystals. We conclude that an 'energetic unit' equates to one helix turn of 6 fructose units per chain as found in one unit cell of the inulin crystal. Each isoform chain comprised progressively more helix turns plus one additional fructose and glucose residues per chain.

    Original languageEnglish
    Pages (from-to)392-397
    Number of pages6
    JournalCARBOHYDRATE POLYMERS
    Volume103
    Issue number1
    DOIs
    Publication statusPublished - 15 Mar 2014

    Keywords

    • Adjuvant
    • Carbohydrate
    • Inulin
    • Isoform
    • Polymorph
    • Vaccine

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