Investigating source water Cryptosporidium concentration, species and infectivity rates during rainfall-runoff in a multi-use catchment

Hayley M. Vial, Brooke A. Swaffer, Brendon J. King, Robert Daly, Jacqueline Frizenschaf, Paul T. Monis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Monitoring source water quality in rivers and catchments remains a key priority not only for catchment managers, but also for water utilities. Here, we applied a best-practice approach to characterise pathogens from the genus Cryptosporidium in rivers used as a drinking water source. Sampling during high flow conditions caused by rainfall run-off determined not only the concentration of Cryptosporidium in water, but also identified the species present and the fraction which remained capable of forming an infection - which to date, has rarely been reported in the literature. The results demonstrate a positive and significant correlation between Cryptosporidium and flow (ρ = 0.756) and turbidity (ρ = 0.631) for all rainfall-runoff events, despite variable source water pathogen concentrations. Twelve Cryptosporidium species/genotypes were identified using molecular techniques, with most Cryptosporidium species detected associated with native and non-native wildlife (comprising 70 % of detections), while livestock-derived Cryptosporidium was detected less frequently (28 % of detections). Importantly, only 3/41 of water samples detected the presence of C. parvum (7 %) and no C. hominis was detected (0 %). Cell culture assays were used to measure the fraction of oocysts and gave an overall infectivity fraction of 3.1 %. To our knowledge, this is the first account which has quantified density, oocyst infectivity and foci species identity from a single environmental water sample.

Original languageEnglish
Title of host publication21st Century Watershed Technology Conference and Workshop 2014
Subtitle of host publicationImproving Water Quality and the Environment
PublisherAmerican Society of Agricultural and Biological Engineers
Pages323-333
Number of pages11
ISBN (Electronic)9781510859616
DOIs
Publication statusPublished - 2014
Externally publishedYes
Event21st Century Watershed Technology Conference and Workshop 2014: Improving Water Quality and the Environment - Hamilton, New Zealand
Duration: 3 Nov 20146 Nov 2014

Publication series

Name21st Century Watershed Technology Conference and Workshop 2014: Improving Water Quality and the Environment

Conference

Conference21st Century Watershed Technology Conference and Workshop 2014: Improving Water Quality and the Environment
CountryNew Zealand
CityHamilton
Period3/11/146/11/14

Keywords

  • Cryptosporidium
  • Drinking water
  • Genotyping
  • Infectivity

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    Vial, H. M., Swaffer, B. A., King, B. J., Daly, R., Frizenschaf, J., & Monis, P. T. (2014). Investigating source water Cryptosporidium concentration, species and infectivity rates during rainfall-runoff in a multi-use catchment. In 21st Century Watershed Technology Conference and Workshop 2014: Improving Water Quality and the Environment (pp. 323-333). (21st Century Watershed Technology Conference and Workshop 2014: Improving Water Quality and the Environment). American Society of Agricultural and Biological Engineers. https://doi.org/10.13031/wtcw.2014-041