Iron uptake by symbiosomes from soybean root nodules

Kristin LeVier, David A. Day, Mary Lou Guerinot

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

To identify possible iron sources for bacteroids in planta, soybean (Glycine max L. Merr.) symbiosomes (consisting of the bacteroid-containing peribacteroid space enclosed by the peribacteroid membrane [PBM]) and bacteroids were assayed for the ability to transport iron supplied as various ferric [Fe(III)]-chelates. Iron presented as a number of Fe(III)-chelates was transported at much higher rates across the PBM than across the bacteroid membranes, suggesting the presence of an iron storage pool in the peribacteroid space. Pulse-chase experiments confirmed the presence of such an iron storage pool. Because the PBM is derived from the plant plasma membrane, we reasoned that it may possess a ferric-chelate reductase activity similar to that present in plant plasma membrane. We detected ferric-chelate reductase activity associated with the PBM and suggest that reduction of Fe(III) to ferrous [Fe(II)] plays a role in the movement of iron into soybean symbiosomes.

Original languageEnglish
Pages (from-to)893-900
Number of pages8
JournalPlant Physiology
Volume111
DOIs
Publication statusPublished - Jul 1996
Externally publishedYes

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