Isolation and characterisation of eighteen microsatellite markers from the sea cucumber, Holothuria scabra (Echinodermata: Holothuriidae)

Alison Fitch, Grant Leeworthy, Xiaxou Li, Will Bowman, Luke Turner, Michael Gardner

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    We isolated 18 new, polymorphic, microsatellite markers from Holothuria scabra, a commercially important species of sea cucumber found throughout the Asia-Pacific region. Next-generation sequencing was used to identify 206 unique loci for which primers were designed. Of these unique loci, we trialled 65 primer pairs in the target species, and 48 (74%) amplified a product of the expected size. Eighteen loci were found to be polymorphic and reliable, and were screened for variation in 50 individuals, from a single population from Croker Island, Northern Territory, Australia. Observed heterozygosity ranged from 0.00 to 0.96 (mean≤0.46) and the number of alleles per locus from 2 to 28 (mean≤9.61). These loci will be useful for the investigation of population structure and mating systems in H. scabra and may also be of use in other holothurian species.

    Original languageEnglish
    Pages (from-to)368-371
    Number of pages4
    JournalAustralian Journal of Zoology
    Volume60
    Issue number6
    DOIs
    Publication statusPublished - 2012

    Keywords

    • GS-FLX
    • next-generation sequencing

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