Isolation and characterisation of microsatellite markers for the dwarf sheoak Allocasuarina humilis (Casuarinaceae), an important understorey species in ecological restoration

Tanya M. Llorens; Shelley McArthur; Michael G. Gardner; David J. Coates; Margaret Byrne

Isolation and characterisation of microsatellite markers for the dwarf sheoak Allocasuarina humilis (Casuarinaceae), an important understorey species in ecological restoration

Tanya M. Llorens, Shelley McArthur, Michael G. Gardner, David J. Coates, Margaret Byrne

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Eight polymorphic microsatellite markers were developed for Allocasuarina humilis (Otto & A.Dietr.) L.A.S.Johnson, a common woody shrub endemic to south-west Australia that is a common component of the understorey across a range of vegetation types. The markers were isolated using 454 shotgun sequencing and tested on 48 individuals from two natural populations. We found six to 19 alleles per locus (mean 12.1) and observed heterozygosity ranged from 0.208 to 0.956 (mean 0.710). The markers will be used to assess population structure and diversity in A. humilis, with the aim of developing appropriate seed sourcing strategies for ecological restoration.

Original language	English
Pages (from-to)	59-62
Number of pages	4
Journal	Muelleria
Volume	34
Publication status	Published - 16 Nov 2015

Keywords

Allocasuarina
Microsatellites
SSRs

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title = "Isolation and characterisation of microsatellite markers for the dwarf sheoak Allocasuarina humilis (Casuarinaceae), an important understorey species in ecological restoration",

abstract = "Eight polymorphic microsatellite markers were developed for Allocasuarina humilis (Otto & A.Dietr.) L.A.S.Johnson, a common woody shrub endemic to south-west Australia that is a common component of the understorey across a range of vegetation types. The markers were isolated using 454 shotgun sequencing and tested on 48 individuals from two natural populations. We found six to 19 alleles per locus (mean 12.1) and observed heterozygosity ranged from 0.208 to 0.956 (mean 0.710). The markers will be used to assess population structure and diversity in A. humilis, with the aim of developing appropriate seed sourcing strategies for ecological restoration.",

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AU - Llorens, Tanya M.

AU - McArthur, Shelley

AU - Gardner, Michael G.

AU - Coates, David J.

AU - Byrne, Margaret

PY - 2015/11/16

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AB - Eight polymorphic microsatellite markers were developed for Allocasuarina humilis (Otto & A.Dietr.) L.A.S.Johnson, a common woody shrub endemic to south-west Australia that is a common component of the understorey across a range of vegetation types. The markers were isolated using 454 shotgun sequencing and tested on 48 individuals from two natural populations. We found six to 19 alleles per locus (mean 12.1) and observed heterozygosity ranged from 0.208 to 0.956 (mean 0.710). The markers will be used to assess population structure and diversity in A. humilis, with the aim of developing appropriate seed sourcing strategies for ecological restoration.

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KW - Microsatellites

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ER -

Isolation and characterisation of microsatellite markers for the dwarf sheoak Allocasuarina humilis (Casuarinaceae), an important understorey species in ecological restoration

Abstract

Keywords

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