Isolation and characterization of microsatellite markers for Passiflora contracta

Ana Caze, Rita Kriedt, Luciano Beheregaray, Sandro Bonatto, Loreta Freitas

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Passiflora contracta Vitta (Passifloraceae) is an endemic species of the Atlantic Rainforest, one of the most species-rich ecoregions in the world, although extremely endangered. We have developed an enriched microsatellite library in order to fine-scale studies of the genetic structure of P. contracta. Twelve pairs of microsatellite primers were designed, and seven loci were successfully amplified and characterized by genotyping two wild populations of P. contracta. All seven loci were polymorphic, with an average number of alleles found being 4.8 and 5 per population. The cross-species transferability was tested using sister species Passiflora ovalis Vell. Ex Roemer. The development of these markers will contribute to the studies of population genetics in P. contracta as well as future studies concerning diversity patterns in the Atlantic Rainforest, and may also help to establish strategies for the conservation of this species.

    Original languageEnglish
    Pages (from-to)11343-11348
    Number of pages6
    JournalInternational Journal of Molecular Sciences
    Volume13
    Issue number9
    DOIs
    Publication statusPublished - 2012

    Fingerprint Dive into the research topics of 'Isolation and characterization of microsatellite markers for Passiflora contracta'. Together they form a unique fingerprint.

  • Cite this