1. A vasopressin (AVP) binding protein was purified from rat liver membranes by an improved method using [125I][d(CH2)5′Sarcosine7]AVP, a selective Vi AVP radioligand and a combination of CHAPS solubilization, gel filtration, lectin affinity and FPLC ion exchange chromatography. 2. The purified protein exhibited a maximum binding activity of 2480 pmol/ mg protein with a KD of 4.5 nmol/ L, which corresponds to a purification of approximately 26 700‐fold. The molecular weight of this protein was 70 000 Da. 3. The binding of [125I][d(CH2)5′Sarcosine7]AVP to the solubilized membranes was dependent on the protein concentration, and was inhibited by the unlabelled peptides [d(CH2)5′Sarcosine7]AVP, AVP, and to a lesser degree by peptides with high V2 receptor affinity, such as 1‐desamino‐D‐AVP and [d(CH2)5′D‐Ileu2‐Ileu4]AVP. 4. In addition, an AVP anti‐idiotypic monoclonal antibody bound to both the partially purified and purified lectin affinity AVP binding protein in a concentration‐dependent manner. These results indicate that the purified protein displays similar characteristics to the liver membrane‐bound AVP V1 receptor.
|Number of pages||8|
|Journal||Clinical and Experimental Pharmacology and Physiology|
|Publication status||Published - Apr 1992|
- arginine vasopressin
- vasopressin antagonist
- vasopressin receptor