@inbook{9a94ee3dbd3c4069b07711c340cb2f66,
title = "Isolation and propagation of lacrimal gland putative epithelial progenitor cells",
abstract = "We present a protocol for isolation of putative epithelial progenitor cells from mouse lacrimal gland (LG) by fluorescence-activated cell sorting (FACS). Isolated LG epithelial progenitor cells can be cultured as 3D reaggregates within extracellular matrix gel or plated as a monolayer. 3D cultures could be maintained for several days and then dissociated with trypsin and plated as monolayer cultures, processed for analysis (e.g., mRNA/protein expression) and/or used for transplantations. Our goal is to provide researchers with a method that can be used as is or modified if isolation of other LG epithelial cell types is required.",
keywords = "3D cultures, Epithelial progenitor cells, FACS, Isolation, Australia",
author = "Makarenkova, {Helen P.} and Robyn Meech",
year = "2019",
month = feb,
day = "21",
doi = "10.1007/978-1-4939-9086-3_12",
language = "English",
isbn = "978-1-4939-9085-6",
volume = "1940",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "169--180",
editor = "Ivan Bertoncello",
booktitle = "Mouse Cell Culture",
address = "United States",
}