Isolation and propagation of lacrimal gland putative epithelial progenitor cells

Helen P. Makarenkova, Robyn Meech

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

We present a protocol for isolation of putative epithelial progenitor cells from mouse lacrimal gland (LG) by fluorescence-activated cell sorting (FACS). Isolated LG epithelial progenitor cells can be cultured as 3D reaggregates within extracellular matrix gel or plated as a monolayer. 3D cultures could be maintained for several days and then dissociated with trypsin and plated as monolayer cultures, processed for analysis (e.g., mRNA/protein expression) and/or used for transplantations. Our goal is to provide researchers with a method that can be used as is or modified if isolation of other LG epithelial cell types is required.

Original languageEnglish
Title of host publicationMouse Cell Culture
Subtitle of host publicationMethods and Protocols
EditorsIvan Bertoncello
Place of PublicationNew York, NY
PublisherHumana Press Inc.
Chapter12
Pages169-180
Number of pages12
Volume1940
ISBN (Electronic)978-1-4939-9086-3
ISBN (Print)978-1-4939-9085-6
DOIs
Publication statusPublished - 21 Feb 2019

Publication series

NameMethods in Molecular Biology
Volume1940
ISSN (Print)1064-3745

Keywords

  • 3D cultures
  • Epithelial progenitor cells
  • FACS
  • Isolation
  • Australia

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  • Cite this

    Makarenkova, H. P., & Meech, R. (2019). Isolation and propagation of lacrimal gland putative epithelial progenitor cells. In I. Bertoncello (Ed.), Mouse Cell Culture: Methods and Protocols (Vol. 1940, pp. 169-180). (Methods in Molecular Biology; Vol. 1940). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9086-3_12