Isolation, characterization and cross-amplification of microsatellite markers for Petunia integrifolia (Solanaceae) complex

Rita Kriedt, Amelia Ramos-Fregonezi, Luciano Beheregaray, Sandro Bonatto, Loreta Freitas

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    8 Citations (Scopus)

    Abstract

    Premise of the study: Microsatellite markers were developed for Petunia integrifolia subsp. depauperata with an intent to clarify taxonomic questions on the P. integrifolia complex, and to identify a purple-flowered parent of P. hybrida. Methods and Results: We characterized 11 microsatellite loci by screening primers developed using an SSR-enriched library. Genotyping of two populations resulted in eight polymorphic loci. Cross-species transferability was tested for other members of the P. integrifolia complex. Conclusions: The development of these markers may contribute to population genetics studies in Petunia, and cross-amplification among related species could be a useful tool for research on hybridization and introgression.

    Original languageEnglish
    Pages (from-to)e277-e279
    Number of pages3
    JournalAmerican Journal of Botany
    Volume98
    Issue number10
    DOIs
    Publication statusPublished - Oct 2011

    Keywords

    • Congeneric transferability
    • Microsatellite
    • Petunia integrifolia complex

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