Isolation of fetal trophoblast cells from peripheral blood of pregnant women

U. W. Mueller, C. S. Hawes, A. E. Wright, E. DeBoni, W. R. Jones, F. A. Firgaira, A. A. Morley, D. R. Turner

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    Abstract

    Fetal trophoblast cells were isolated from maternal peripheral blood by means of murine monoclonal antibodies of high specificity and affinity for human syncytiotrophoblast and nonvillous cytotrophoblast cells. The cells were isolated in sufficient numbers to allow polymerase chain reaction (PCR) amplification of the Y-chromosome-specific DNA sequence from the peripheral blood of thirteen pregnant women. The fetal sex predicted by PCR analysis of the isolated trophoblast cells accorded with that ascertained by karyotyping of chorionic villus samples in eleven of twelve women studied in early pregnancy and with the sex of the baby on delivery in one woman studied at 34 weeks' gestation. Isolation of these fetal cells could allow non-invasive diagnosis of a wide range of inherited disorders.

    Original languageEnglish
    Pages (from-to)197-200
    Number of pages4
    JournalThe Lancet
    Volume336
    Issue number8709
    DOIs
    Publication statusPublished - 28 Jul 1990

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    Mueller, U. W., Hawes, C. S., Wright, A. E., DeBoni, E., Jones, W. R., Firgaira, F. A., Morley, A. A., & Turner, D. R. (1990). Isolation of fetal trophoblast cells from peripheral blood of pregnant women. The Lancet, 336(8709), 197-200. https://doi.org/10.1016/0140-6736(90)91731-O