Isolation of microsatellites in a parthenogenetic lizard Menetia greyii (Scincidae) and their utility in sexual species of the Menetia greyii complex

M. G. Gardner; K. Ottewell; M. Adams

doi:10.1111/j.1471-8286.2004.00622.x

Isolation of microsatellites in a parthenogenetic lizard Menetia greyii (Scincidae) and their utility in sexual species of the Menetia greyii complex

M. G. Gardner, K. Ottewell, M. Adams

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Tetra and tri-nucleotide microsatellite loci were isolated from a parthenogenetic form of the Australian lizard Menetia greyii, using an enrichment method with polymerase chain reaction (PCR)-based colony screening. Primers for 23 loci were designed and 11 of these loci amplified well in a panel of 10 parthenogenetic individuals from a single population. Seven loci were further characterized and six successfully amplified and were polymorphic in all five sexual species of the Menetia greyii complex they were tested in. These loci will be used to investigate the parental ancestry and clonal diversity of various parthenogenetic forms within the Menetia greyii species complex.

Original language	English
Pages (from-to)	219-221
Number of pages	3
Journal	Molecular Ecology Notes
Volume	4
Issue number	2
DOIs	https://doi.org/10.1111/j.1471-8286.2004.00622.x
Publication status	Published - Jun 2004
Externally published	Yes

Keywords

Enrichment
Menetia greyii
Microsatellite
Parthenogenesis
Skink
Unisexual

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10.1111/j.1471-8286.2004.00622.xLicence: In Copyright

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AU - Adams, M.

PY - 2004/6

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AB - Tetra and tri-nucleotide microsatellite loci were isolated from a parthenogenetic form of the Australian lizard Menetia greyii, using an enrichment method with polymerase chain reaction (PCR)-based colony screening. Primers for 23 loci were designed and 11 of these loci amplified well in a panel of 10 parthenogenetic individuals from a single population. Seven loci were further characterized and six successfully amplified and were polymorphic in all five sexual species of the Menetia greyii complex they were tested in. These loci will be used to investigate the parental ancestry and clonal diversity of various parthenogenetic forms within the Menetia greyii species complex.

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Isolation of microsatellites in a parthenogenetic lizard Menetia greyii (Scincidae) and their utility in sexual species of the Menetia greyii complex

Abstract

Keywords

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