Isolation of microsatellites in a parthenogenetic lizard Menetia greyii (Scincidae) and their utility in sexual species of the Menetia greyii complex

M. G. Gardner, K. Ottewell, M. Adams

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Tetra and tri-nucleotide microsatellite loci were isolated from a parthenogenetic form of the Australian lizard Menetia greyii, using an enrichment method with polymerase chain reaction (PCR)-based colony screening. Primers for 23 loci were designed and 11 of these loci amplified well in a panel of 10 parthenogenetic individuals from a single population. Seven loci were further characterized and six successfully amplified and were polymorphic in all five sexual species of the Menetia greyii complex they were tested in. These loci will be used to investigate the parental ancestry and clonal diversity of various parthenogenetic forms within the Menetia greyii species complex.

Original languageEnglish
Pages (from-to)219-221
Number of pages3
JournalMolecular Ecology Notes
Volume4
Issue number2
DOIs
Publication statusPublished - Jun 2004
Externally publishedYes

Keywords

  • Enrichment
  • Menetia greyii
  • Microsatellite
  • Parthenogenesis
  • Skink
  • Unisexual

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