Isolation of villous cytotrophoblasts from second trimester human placentas

Tanja Jankovic-Karasoulos, Dale McAninch, Dylan McCullough, Rebecca L. Wilson, Tina Bianco-Miotto, Claire T. Roberts

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Human placental functional studies are often performed using immortalised trophoblast cells and cell lines established from human choriocarcinomas, which, while practical, may not truly reflect trophoblast function in vivo. Primary trophoblast cultures derived from human placentas following pregnancy termination or delivery are more clinically relevant, but trophoblast isolation protocols are only available for 1st trimester and term placental tissues. Here we report a method for isolation and purification of primary villous cytotrophoblasts from 2nd trimester human placentas, that yields >99% trophoblast purity as shown by immunofluorescence.

Original languageEnglish
Pages (from-to)55-58
Number of pages4
JournalPLACENTA
Volume74
DOIs
Publication statusPublished - 15 Dec 2018
Externally publishedYes

Keywords

  • Placenta
  • Primary cell isolation
  • Second trimester
  • Trophoblasts

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