Isolation via 454 sequencing, and characterisation of microsatellite markers for the Pilbara endemic Acacia atkinsiana (Fabaceae)

Esther Levy; Margaret Byrne; David Coates; Stephen van Leeuwen; Shelley McArthur; Bronwyn Macdonald; Michael Gardner

doi:10.1007/s12686-014-0146-3

Isolation via 454 sequencing, and characterisation of microsatellite markers for the Pilbara endemic Acacia atkinsiana (Fabaceae)

Esther Levy, Margaret Byrne, David Coates, Stephen van Leeuwen, Shelley McArthur, Bronwyn Macdonald, Michael Gardner

Research output: Contribution to journal › Letter › peer-review

4 Citations (Scopus)

Abstract

We isolated 11 new polymorphic microsatellite markers from Acacia atkinsiana, a plant species endemic to the Pilbara region in Western Australia. Next generation (454) sequencing was used to identify 28 microsatellite markers that were trialled in individuals across the species range. Characterisation of 11 of these loci in 24 individuals from a single population yielded between two and nine alleles per locus, and observed heterozygosity ranged from 0.042 to 0.958. Four loci that amplified in A. atkinsiana were also successfully amplified and polymorphic in Acacia ancistrocarpa. These loci will be valuable for assessing population genetic structure in A. atkinsiana and identifying seed sources for rehabilitation of disturbed sites.

Original language	English
Pages (from-to)	585-587
Number of pages	3
Journal	Conservation Genetics Resources
Volume	6
Issue number	3
DOIs	https://doi.org/10.1007/s12686-014-0146-3
Publication status	Published - Aug 2014

Keywords

454 GS-FLX
Acacia atkinsiana
Microsatellites
Revegetation
Shotgun sequencing

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10.1007/s12686-014-0146-3

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title = "Isolation via 454 sequencing, and characterisation of microsatellite markers for the Pilbara endemic Acacia atkinsiana (Fabaceae)",

abstract = "We isolated 11 new polymorphic microsatellite markers from Acacia atkinsiana, a plant species endemic to the Pilbara region in Western Australia. Next generation (454) sequencing was used to identify 28 microsatellite markers that were trialled in individuals across the species range. Characterisation of 11 of these loci in 24 individuals from a single population yielded between two and nine alleles per locus, and observed heterozygosity ranged from 0.042 to 0.958. Four loci that amplified in A. atkinsiana were also successfully amplified and polymorphic in Acacia ancistrocarpa. These loci will be valuable for assessing population genetic structure in A. atkinsiana and identifying seed sources for rehabilitation of disturbed sites.",

keywords = "454 GS-FLX, Acacia atkinsiana, Microsatellites, Revegetation, Shotgun sequencing",

author = "Esther Levy and Margaret Byrne and David Coates and {van Leeuwen}, Stephen and Shelley McArthur and Bronwyn Macdonald and Michael Gardner",

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doi = "10.1007/s12686-014-0146-3",

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T1 - Isolation via 454 sequencing, and characterisation of microsatellite markers for the Pilbara endemic Acacia atkinsiana (Fabaceae)

AU - Levy, Esther

AU - Byrne, Margaret

AU - Coates, David

AU - van Leeuwen, Stephen

AU - McArthur, Shelley

AU - Macdonald, Bronwyn

AU - Gardner, Michael

PY - 2014/8

Y1 - 2014/8

N2 - We isolated 11 new polymorphic microsatellite markers from Acacia atkinsiana, a plant species endemic to the Pilbara region in Western Australia. Next generation (454) sequencing was used to identify 28 microsatellite markers that were trialled in individuals across the species range. Characterisation of 11 of these loci in 24 individuals from a single population yielded between two and nine alleles per locus, and observed heterozygosity ranged from 0.042 to 0.958. Four loci that amplified in A. atkinsiana were also successfully amplified and polymorphic in Acacia ancistrocarpa. These loci will be valuable for assessing population genetic structure in A. atkinsiana and identifying seed sources for rehabilitation of disturbed sites.

AB - We isolated 11 new polymorphic microsatellite markers from Acacia atkinsiana, a plant species endemic to the Pilbara region in Western Australia. Next generation (454) sequencing was used to identify 28 microsatellite markers that were trialled in individuals across the species range. Characterisation of 11 of these loci in 24 individuals from a single population yielded between two and nine alleles per locus, and observed heterozygosity ranged from 0.042 to 0.958. Four loci that amplified in A. atkinsiana were also successfully amplified and polymorphic in Acacia ancistrocarpa. These loci will be valuable for assessing population genetic structure in A. atkinsiana and identifying seed sources for rehabilitation of disturbed sites.

KW - 454 GS-FLX

KW - Acacia atkinsiana

KW - Microsatellites

KW - Revegetation

KW - Shotgun sequencing

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U2 - 10.1007/s12686-014-0146-3

DO - 10.1007/s12686-014-0146-3

M3 - Letter

SN - 1877-7252

VL - 6

SP - 585

EP - 587

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - 3

ER -

Isolation via 454 sequencing, and characterisation of microsatellite markers for the Pilbara endemic Acacia atkinsiana (Fabaceae)

Abstract

Keywords

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