Isolation via 454 sequencing and characterisation of microsatellites for Acacia montana (Fabaceae), Mallee wattle: an endemic shrub from southeastern Australia

Tara Hopley, Linda Broadhurst, Michael Gardner

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    We developed eight new polymorphic microsatellite markers for Acacia montana, a bushy shrub from south-eastern Australia used in revegetation projects. Initially, primers were designed for 32 loci identified from 454 shotgun sequencing. Eight loci amplified successfully and were polymorphic when screened on 40 individuals, with observed heterozygosity ranging from 0.250 to 0.789 and the number of alleles per locus from 4 to 13. These loci will be useful in understanding the genetic variation and structure of A. montana in its current fragmented range.

    Original languageEnglish
    Pages (from-to)171-172
    Number of pages2
    JournalConservation Genetics Resources
    Volume7
    Issue number1
    DOIs
    Publication statusPublished - 2015

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