Isolation via 454 sequencing and characterisation of microsatellites for Acacia montana (Fabaceae), Mallee wattle: an endemic shrub from southeastern Australia

Tara Hopley; Linda Broadhurst; Michael Gardner

doi:10.1007/s12686-014-0321-6

Isolation via 454 sequencing and characterisation of microsatellites for Acacia montana (Fabaceae), Mallee wattle: an endemic shrub from southeastern Australia

Tara Hopley, Linda Broadhurst, Michael Gardner

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We developed eight new polymorphic microsatellite markers for Acacia montana, a bushy shrub from south-eastern Australia used in revegetation projects. Initially, primers were designed for 32 loci identified from 454 shotgun sequencing. Eight loci amplified successfully and were polymorphic when screened on 40 individuals, with observed heterozygosity ranging from 0.250 to 0.789 and the number of alleles per locus from 4 to 13. These loci will be useful in understanding the genetic variation and structure of A. montana in its current fragmented range.

Original language	English
Pages (from-to)	171-172
Number of pages	2
Journal	Conservation Genetics Resources
Volume	7
Issue number	1
DOIs	https://doi.org/10.1007/s12686-014-0321-6
Publication status	Published - Mar 2015

Keywords

454 GS-FLX
Acacia montana
Mallee wattle
Microsatellites
Seed production area
Shotgun sequencing

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10.1007/s12686-014-0321-6

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abstract = "We developed eight new polymorphic microsatellite markers for Acacia montana, a bushy shrub from south-eastern Australia used in revegetation projects. Initially, primers were designed for 32 loci identified from 454 shotgun sequencing. Eight loci amplified successfully and were polymorphic when screened on 40 individuals, with observed heterozygosity ranging from 0.250 to 0.789 and the number of alleles per locus from 4 to 13. These loci will be useful in understanding the genetic variation and structure of A. montana in its current fragmented range.",

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AU - Gardner, Michael

PY - 2015/3

Y1 - 2015/3

N2 - We developed eight new polymorphic microsatellite markers for Acacia montana, a bushy shrub from south-eastern Australia used in revegetation projects. Initially, primers were designed for 32 loci identified from 454 shotgun sequencing. Eight loci amplified successfully and were polymorphic when screened on 40 individuals, with observed heterozygosity ranging from 0.250 to 0.789 and the number of alleles per locus from 4 to 13. These loci will be useful in understanding the genetic variation and structure of A. montana in its current fragmented range.

AB - We developed eight new polymorphic microsatellite markers for Acacia montana, a bushy shrub from south-eastern Australia used in revegetation projects. Initially, primers were designed for 32 loci identified from 454 shotgun sequencing. Eight loci amplified successfully and were polymorphic when screened on 40 individuals, with observed heterozygosity ranging from 0.250 to 0.789 and the number of alleles per locus from 4 to 13. These loci will be useful in understanding the genetic variation and structure of A. montana in its current fragmented range.

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KW - Acacia montana

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KW - Microsatellites

KW - Seed production area

KW - Shotgun sequencing

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Isolation via 454 sequencing and characterisation of microsatellites for Acacia montana (Fabaceae), Mallee wattle: an endemic shrub from southeastern Australia

Abstract

Keywords

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