Isolation, via 454 sequencing, characterisation and transferability of twelve microsatellite loci for Hydrophis spiralis, the yellow sea snake (Serpentes: Elapidae)

Kate Sanders, Michael Gardner

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    We isolated twelve new polymorphic microsatellite markers from the viviparous sea snake Hydrophis spiralis (Elapidae: Hydrophiinae) from West Java, Indonesia. Initially, 454 shotgun sequencing (GS-FLX) was used to identify 555 loci. Of these we trialled 30 loci in 2 target species, H. cyanocinctus and H. coggeri (closely related to H. spiralis), and 24 (80 %) amplified a product of expected size. We subsequently screened these loci for cross-amplification and polymorphism in 41 individuals of 4 species (H. cyanocinctus, H. coggeri, H. melanocephalus and H. parviceps). Twelve loci were polymorphic with observed within-species heterozygosity ranging from 0 to 1 (mean 0. 48), and the number of alleles per locus per species ranging from 1 to 9 (average of 3. 4). These loci will be useful in studies of sea snake conservation genetics and speciation dynamics.

    Original languageEnglish
    Pages (from-to)1101-1104
    Number of pages4
    JournalConservation Genetics Resources
    Volume4
    Issue number4
    DOIs
    Publication statusPublished - Dec 2012

    Keywords

    • 454 GS-FLX
    • Cross-species transferability
    • Hydrophis spiralis
    • IUCN data deficient
    • Microsatellites
    • Shotgun sequencing
    • Yellow sea snake

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