Juxtacellular Neuronal Labelling, Physiological Characterization and Phenotypic Identification of Single Neurons In Vivo

Anthony Verberne; Ida Llewellyn-Smith

doi:10.1007/978-1-62703-233-9-10

Juxtacellular Neuronal Labelling, Physiological Characterization and Phenotypic Identification of Single Neurons In Vivo

Anthony Verberne, Ida Llewellyn-Smith

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

2 Citations (Scopus)

Abstract

Characterisation of the phenotype and physiological responsiveness of a neuron and identification of its innervation target(s) are important objectives for neuroscientists. This chapter describes the technique of juxtacellular neuronal labelling and presents procedures for the light and electron microscopic visualisation of labelled neurons and the identification of their neurochemical phenotypes and inputs. For those with little experience in extracellular neuronal recording, a detailed description of the required pieces of equipment and how they are configured is also provided.

Original language	English
Title of host publication	Stimulation and Inhibition of Neurons
Editors	Paul Pilowsky, Melissa Farnham, Angelina Fong
Publisher	HUMANA PRESS
Pages	167-186
Number of pages	20
ISBN (Print)	9781627032322
DOIs	https://doi.org/10.1007/978-1-62703-233-9-10
Publication status	Published - 2013

Publication series

Name	Neuromethods
Volume	78
ISSN (Print)	0893-2336
ISSN (Electronic)	1940-6045

Keywords

Electrophysiology
Extracellular
Immunohistochemistry
Neurochemistry
Ultrastructure

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10.1007/978-1-62703-233-9-10

Cite this

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abstract = "Characterisation of the phenotype and physiological responsiveness of a neuron and identification of its innervation target(s) are important objectives for neuroscientists. This chapter describes the technique of juxtacellular neuronal labelling and presents procedures for the light and electron microscopic visualisation of labelled neurons and the identification of their neurochemical phenotypes and inputs. For those with little experience in extracellular neuronal recording, a detailed description of the required pieces of equipment and how they are configured is also provided.",

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Juxtacellular Neuronal Labelling, Physiological Characterization and Phenotypic Identification of Single Neurons In Vivo. / Verberne, Anthony; Llewellyn-Smith, Ida.

Stimulation and Inhibition of Neurons. ed. / Paul Pilowsky; Melissa Farnham; Angelina Fong. HUMANA PRESS, 2013. p. 167-186 (Neuromethods; Vol. 78).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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AU - Verberne, Anthony

AU - Llewellyn-Smith, Ida

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N2 - Characterisation of the phenotype and physiological responsiveness of a neuron and identification of its innervation target(s) are important objectives for neuroscientists. This chapter describes the technique of juxtacellular neuronal labelling and presents procedures for the light and electron microscopic visualisation of labelled neurons and the identification of their neurochemical phenotypes and inputs. For those with little experience in extracellular neuronal recording, a detailed description of the required pieces of equipment and how they are configured is also provided.

AB - Characterisation of the phenotype and physiological responsiveness of a neuron and identification of its innervation target(s) are important objectives for neuroscientists. This chapter describes the technique of juxtacellular neuronal labelling and presents procedures for the light and electron microscopic visualisation of labelled neurons and the identification of their neurochemical phenotypes and inputs. For those with little experience in extracellular neuronal recording, a detailed description of the required pieces of equipment and how they are configured is also provided.

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KW - Extracellular

KW - Immunohistochemistry

KW - Neurochemistry

KW - Ultrastructure

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M3 - Chapter

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T3 - Neuromethods

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EP - 186

BT - Stimulation and Inhibition of Neurons

A2 - Pilowsky, Paul

A2 - Farnham, Melissa

A2 - Fong, Angelina

PB - HUMANA PRESS

ER -

Juxtacellular Neuronal Labelling, Physiological Characterization and Phenotypic Identification of Single Neurons In Vivo

Abstract

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Keywords

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