Lack of correlation between in vitro and in vivo effects of low density lipoprotein on the inflammatory activity of monosodium urate crystals

T. P. Gordon; P. Clifton; M. J. James; P. J. Roberts-Thomson

doi:10.1136/ard.45.8.673

Lack of correlation between in vitro and in vivo effects of low density lipoprotein on the inflammatory activity of monosodium urate crystals

T. P. Gordon
, P. Clifton
, M. J. James
, P. J. Roberts-Thomson

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The effect of coating monosodium urate crystals (MSU) with low density lipoprotein (LDL), postulated previously as a major regulator of gouty inflammation, was studied in a neutrophil chemiluminesce (CL) assay and an air pouch model of inflammation induced by MSU. LDL crystalline coating abrogated the neutrophil CL response but, in contrast, had no inhibitory effect on leucocyte accumulation, levels of the prostaglandin (PG) metabolite 6-keto-PGF(1α), and exudation of plasma proteins in the in vivo model. This latter observation raises doubts about the postulated physiological role of LDL in terminating the acute gouty attack.

Original language	English
Pages (from-to)	673-676
Number of pages	4
Journal	Annals of the Rheumatic Diseases
Volume	45
Issue number	8
DOIs	https://doi.org/10.1136/ard.45.8.673
Publication status	Published - 1986
Externally published	Yes

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title = "Lack of correlation between in vitro and in vivo effects of low density lipoprotein on the inflammatory activity of monosodium urate crystals",

abstract = "The effect of coating monosodium urate crystals (MSU) with low density lipoprotein (LDL), postulated previously as a major regulator of gouty inflammation, was studied in a neutrophil chemiluminesce (CL) assay and an air pouch model of inflammation induced by MSU. LDL crystalline coating abrogated the neutrophil CL response but, in contrast, had no inhibitory effect on leucocyte accumulation, levels of the prostaglandin (PG) metabolite 6-keto-PGF(1α), and exudation of plasma proteins in the in vivo model. This latter observation raises doubts about the postulated physiological role of LDL in terminating the acute gouty attack.",

author = "Gordon, \{T. P.\} and P. Clifton and James, \{M. J.\} and Roberts-Thomson, \{P. J.\}",

year = "1986",

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T1 - Lack of correlation between in vitro and in vivo effects of low density lipoprotein on the inflammatory activity of monosodium urate crystals

AU - Gordon, T. P.

AU - Clifton, P.

AU - James, M. J.

AU - Roberts-Thomson, P. J.

PY - 1986

Y1 - 1986

N2 - The effect of coating monosodium urate crystals (MSU) with low density lipoprotein (LDL), postulated previously as a major regulator of gouty inflammation, was studied in a neutrophil chemiluminesce (CL) assay and an air pouch model of inflammation induced by MSU. LDL crystalline coating abrogated the neutrophil CL response but, in contrast, had no inhibitory effect on leucocyte accumulation, levels of the prostaglandin (PG) metabolite 6-keto-PGF(1α), and exudation of plasma proteins in the in vivo model. This latter observation raises doubts about the postulated physiological role of LDL in terminating the acute gouty attack.

AB - The effect of coating monosodium urate crystals (MSU) with low density lipoprotein (LDL), postulated previously as a major regulator of gouty inflammation, was studied in a neutrophil chemiluminesce (CL) assay and an air pouch model of inflammation induced by MSU. LDL crystalline coating abrogated the neutrophil CL response but, in contrast, had no inhibitory effect on leucocyte accumulation, levels of the prostaglandin (PG) metabolite 6-keto-PGF(1α), and exudation of plasma proteins in the in vivo model. This latter observation raises doubts about the postulated physiological role of LDL in terminating the acute gouty attack.

UR - https://www.scopus.com/pages/publications/0022536648

U2 - 10.1136/ard.45.8.673

DO - 10.1136/ard.45.8.673

M3 - Article

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SN - 0003-4967

VL - 45

SP - 673

EP - 676

JO - Annals of the Rheumatic Diseases

JF - Annals of the Rheumatic Diseases

IS - 8

ER -

Lack of correlation between in vitro and in vivo effects of low density lipoprotein on the inflammatory activity of monosodium urate crystals

Abstract

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