Large scale, rapid preparation of functional mesophyll chloroplasts from Zea mays and other C4 species

C. L.D. Jenkins, V. J. Russ

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

A procedure for the rapid, large scale preparation of leaf mesophyll chloroplasts from Zea mays by mechanical methods is described. Centrifugation through a Percoll density layer is used as a final purification step, yielding chloroplasts showing 80-90% intactness and high rates of substrate-dependent oxygen evolution under illumination. Thus, these chloroplasts are of similar functional integrity to those prepared from C4 leaf mesophyll protoplasts but the method allows a considerable saving in time and greater yields. The method has been found suitable for mesophyll chloroplast preparation from several other C4-species.

Original languageEnglish
Pages (from-to)19-24
Number of pages6
JournalPlant Science Letters
Volume35
Issue number1
DOIs
Publication statusPublished - 16 May 1984
Externally publishedYes

Keywords

  • C mesophyll chloroplasts
  • C-photosynthesis
  • chloroplast activities
  • chloroplast preparation
  • Zea mays

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