Abstract
A procedure for the rapid, large scale preparation of leaf mesophyll chloroplasts from Zea mays by mechanical methods is described. Centrifugation through a Percoll density layer is used as a final purification step, yielding chloroplasts showing 80-90% intactness and high rates of substrate-dependent oxygen evolution under illumination. Thus, these chloroplasts are of similar functional integrity to those prepared from C4 leaf mesophyll protoplasts but the method allows a considerable saving in time and greater yields. The method has been found suitable for mesophyll chloroplast preparation from several other C4-species.
Original language | English |
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Pages (from-to) | 19-24 |
Number of pages | 6 |
Journal | Plant Science Letters |
Volume | 35 |
Issue number | 1 |
DOIs | |
Publication status | Published - 16 May 1984 |
Externally published | Yes |
Keywords
- C mesophyll chloroplasts
- C-photosynthesis
- chloroplast activities
- chloroplast preparation
- Zea mays