TY - JOUR
T1 - Light-assisted small-molecule screening against protein kinases
AU - Inglés-Prieto, Álvaro
AU - Reichhart, Eva
AU - Muellner, Markus K.
AU - Nowak, Matthias
AU - Nijman, Sebastian M.B.
AU - Grusch, Michael
AU - Janovjak, Harald
PY - 2015/12
Y1 - 2015/12
N2 - High-throughput live-cell screens are intricate elements of systems biology studies and drug discovery pipelines. Here, we demonstrate an optogenetics-assisted method that avoids the need for chemical activators and reporters, reduces the number of operational steps and increases information content in a cell-based small-molecule screen against human protein kinases, including an orphan receptor tyrosine kinase. This blueprint for all-optical screening can be adapted to many drug targets and cellular processes.
AB - High-throughput live-cell screens are intricate elements of systems biology studies and drug discovery pipelines. Here, we demonstrate an optogenetics-assisted method that avoids the need for chemical activators and reporters, reduces the number of operational steps and increases information content in a cell-based small-molecule screen against human protein kinases, including an orphan receptor tyrosine kinase. This blueprint for all-optical screening can be adapted to many drug targets and cellular processes.
UR - http://www.scopus.com/inward/record.url?scp=84947721213&partnerID=8YFLogxK
U2 - 10.1038/nchembio.1933
DO - 10.1038/nchembio.1933
M3 - Article
C2 - 26457372
AN - SCOPUS:84947721213
SN - 1552-4450
VL - 11
SP - 952
EP - 954
JO - Nature Chemical Biology
JF - Nature Chemical Biology
IS - 12
ER -