Light-assisted small-molecule screening against protein kinases

Álvaro Inglés-Prieto, Eva Reichhart, Markus K. Muellner, Matthias Nowak, Sebastian M.B. Nijman, Michael Grusch, Harald Janovjak

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

High-throughput live-cell screens are intricate elements of systems biology studies and drug discovery pipelines. Here, we demonstrate an optogenetics-assisted method that avoids the need for chemical activators and reporters, reduces the number of operational steps and increases information content in a cell-based small-molecule screen against human protein kinases, including an orphan receptor tyrosine kinase. This blueprint for all-optical screening can be adapted to many drug targets and cellular processes.

Original languageEnglish
Pages (from-to)952-954
Number of pages3
JournalNature Chemical Biology
Volume11
Issue number12
DOIs
Publication statusPublished - Dec 2015
Externally publishedYes

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