Light-assisted small-molecule screening against protein kinases

Álvaro Inglés-Prieto; Eva Reichhart; Markus K. Muellner; Matthias Nowak; Sebastian M.B. Nijman; Michael Grusch; Harald Janovjak

doi:10.1038/nchembio.1933

Light-assisted small-molecule screening against protein kinases

Álvaro Inglés-Prieto
, Eva Reichhart
, Markus K. Muellner
, Matthias Nowak
, Sebastian M.B. Nijman
, Michael Grusch
, Harald Janovjak

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

High-throughput live-cell screens are intricate elements of systems biology studies and drug discovery pipelines. Here, we demonstrate an optogenetics-assisted method that avoids the need for chemical activators and reporters, reduces the number of operational steps and increases information content in a cell-based small-molecule screen against human protein kinases, including an orphan receptor tyrosine kinase. This blueprint for all-optical screening can be adapted to many drug targets and cellular processes.

Original language	English
Pages (from-to)	952-954
Number of pages	3
Journal	Nature Chemical Biology
Volume	11
Issue number	12
DOIs	https://doi.org/10.1038/nchembio.1933
Publication status	Published - Dec 2015
Externally published	Yes

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AU - Inglés-Prieto, Álvaro

AU - Reichhart, Eva

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AU - Nowak, Matthias

AU - Nijman, Sebastian M.B.

AU - Grusch, Michael

AU - Janovjak, Harald

PY - 2015/12

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AB - High-throughput live-cell screens are intricate elements of systems biology studies and drug discovery pipelines. Here, we demonstrate an optogenetics-assisted method that avoids the need for chemical activators and reporters, reduces the number of operational steps and increases information content in a cell-based small-molecule screen against human protein kinases, including an orphan receptor tyrosine kinase. This blueprint for all-optical screening can be adapted to many drug targets and cellular processes.

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Light-assisted small-molecule screening against protein kinases

Abstract

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