Linear and branched polyacrylates as a delivery platform for peptide-based vaccines

Saranya Chandrudu; Stacey Bartlett; Zeinab G. Khalil; Zhongfan Jia; Waleed M. Hussein; Robert J. Capon; Michael R. Batzloff; Michael F. Good; Michael J. Monteiro; Mariusz Skwarczynski; Istvan Toth

doi:10.4155/tde-2016-0037

Linear and branched polyacrylates as a delivery platform for peptide-based vaccines

Saranya Chandrudu, Stacey Bartlett, Zeinab G. Khalil, Zhongfan Jia, Waleed M. Hussein, Robert J. Capon, Michael R. Batzloff, Michael F. Good, Michael J. Monteiro, Mariusz Skwarczynski, Istvan Toth

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Aim: Peptide-based vaccines are designed to carry the minimum required antigen to trigger the desired immune responses; however, they are usually poorly immunogenic and require appropriate delivery system. Results: Peptides, B-cell epitope (J14) derived from group A streptococcus M-protein and universal T-helper (PADRE) epitope, were conjugated to a variety of linear and branched polyacrylates. All produced conjugates formed submicron-sized particles and induced a high level of IgG titres in mice after subcutaneous immunization. These polymer-peptide conjugates demonstrated high opsonization capacity against group A streptococcus clinical isolates. Conclusion: We have successfully demonstrated that submicron-sized polymer-peptide conjugates were capable of inducing strong humoral immune responses after single immunization.

Original language	English
Pages (from-to)	601-609
Number of pages	9
Journal	Therapeutic Delivery
Volume	7
Issue number	9
DOIs	https://doi.org/10.4155/tde-2016-0037
Publication status	Published - Sep 2016
Externally published	Yes

Keywords

clinical isolates
group A streptococcus
nanoparticles
opsonization
peptide vaccine
polyacrylates
polymer-peptide conjugate
single immunization
vaccine delivery

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title = "Linear and branched polyacrylates as a delivery platform for peptide-based vaccines",

abstract = "Aim: Peptide-based vaccines are designed to carry the minimum required antigen to trigger the desired immune responses; however, they are usually poorly immunogenic and require appropriate delivery system. Results: Peptides, B-cell epitope (J14) derived from group A streptococcus M-protein and universal T-helper (PADRE) epitope, were conjugated to a variety of linear and branched polyacrylates. All produced conjugates formed submicron-sized particles and induced a high level of IgG titres in mice after subcutaneous immunization. These polymer-peptide conjugates demonstrated high opsonization capacity against group A streptococcus clinical isolates. Conclusion: We have successfully demonstrated that submicron-sized polymer-peptide conjugates were capable of inducing strong humoral immune responses after single immunization.",

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doi = "10.4155/tde-2016-0037",

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Linear and branched polyacrylates as a delivery platform for peptide-based vaccines. / Chandrudu, Saranya; Bartlett, Stacey; Khalil, Zeinab G.; Jia, Zhongfan; Hussein, Waleed M.; Capon, Robert J.; Batzloff, Michael R.; Good, Michael F.; Monteiro, Michael J.; Skwarczynski, Mariusz; Toth, Istvan.

In: Therapeutic Delivery, Vol. 7, No. 9, 09.2016, p. 601-609.

Research output: Contribution to journal › Article › peer-review

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T1 - Linear and branched polyacrylates as a delivery platform for peptide-based vaccines

AU - Chandrudu, Saranya

AU - Bartlett, Stacey

AU - Khalil, Zeinab G.

AU - Jia, Zhongfan

AU - Hussein, Waleed M.

AU - Capon, Robert J.

AU - Batzloff, Michael R.

AU - Good, Michael F.

AU - Monteiro, Michael J.

AU - Skwarczynski, Mariusz

AU - Toth, Istvan

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AB - Aim: Peptide-based vaccines are designed to carry the minimum required antigen to trigger the desired immune responses; however, they are usually poorly immunogenic and require appropriate delivery system. Results: Peptides, B-cell epitope (J14) derived from group A streptococcus M-protein and universal T-helper (PADRE) epitope, were conjugated to a variety of linear and branched polyacrylates. All produced conjugates formed submicron-sized particles and induced a high level of IgG titres in mice after subcutaneous immunization. These polymer-peptide conjugates demonstrated high opsonization capacity against group A streptococcus clinical isolates. Conclusion: We have successfully demonstrated that submicron-sized polymer-peptide conjugates were capable of inducing strong humoral immune responses after single immunization.

KW - clinical isolates

KW - group A streptococcus

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KW - opsonization

KW - peptide vaccine

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KW - single immunization

KW - vaccine delivery

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Linear and branched polyacrylates as a delivery platform for peptide-based vaccines

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