Abstract
Aim: Peptide-based vaccines are designed to carry the minimum required antigen to trigger the desired immune responses; however, they are usually poorly immunogenic and require appropriate delivery system. Results: Peptides, B-cell epitope (J14) derived from group A streptococcus M-protein and universal T-helper (PADRE) epitope, were conjugated to a variety of linear and branched polyacrylates. All produced conjugates formed submicron-sized particles and induced a high level of IgG titres in mice after subcutaneous immunization. These polymer-peptide conjugates demonstrated high opsonization capacity against group A streptococcus clinical isolates. Conclusion: We have successfully demonstrated that submicron-sized polymer-peptide conjugates were capable of inducing strong humoral immune responses after single immunization.
Original language | English |
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Pages (from-to) | 601-609 |
Number of pages | 9 |
Journal | Therapeutic Delivery |
Volume | 7 |
Issue number | 9 |
DOIs | |
Publication status | Published - Sep 2016 |
Externally published | Yes |
Keywords
- clinical isolates
- group A streptococcus
- nanoparticles
- opsonization
- peptide vaccine
- polyacrylates
- polymer-peptide conjugate
- single immunization
- vaccine delivery