Linoleic Acid:Dihomo-γ-Linolenic Acid Ratio Predicts the Efficacy of Zn-Biofortified Wheat in Chicken (Gallus gallus)

Marija Knez; Elad Tako; Raymond Glahn; Nikolai Kolba; Emma de Courcy-Ireland; James Stangoulis

doi:10.1021/acs.jafc.7b04905

Linoleic Acid:Dihomo-γ-Linolenic Acid Ratio Predicts the Efficacy of Zn-Biofortified Wheat in Chicken (Gallus gallus)

Marija Knez, Elad Tako, Raymond Glahn, Nikolai Kolba, Emma de Courcy-Ireland, James Stangoulis

College of Science and Engineering

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The amount of Zn absorbed from Zn-biofortified wheat material has been determined using an in vivo model of Zn absorption. The erythrocyte linoleic:dihomo -γ-linolenic acid (LA:DGLA) ratio was used as a biomarker of Zn status. Two groups of chickens (n = 15) were fed different diets: a high-Zn (46.5 μg Zn g^-1) and a low-Zn wheat-based diet (32.8 μg Zn g^-1). Dietary Zn intakes, body weight, serum Zn, and the erythrocyte fatty acid profile were measured, and tissues were taken for gene expression analysis. Serum Zn concentrations were greater in the high Zn group (p < 0.05). Duodenal mRNA expression of various Zn transporters demonstrated expression upregulation in the birds fed a low Zn diet (n = 15, p < 0.05). The LA:DGLA ratio was higher in the birds fed the low Zn diet (p < 0.05). The higher amount of Zn in the biofortified wheat resulted in a greater Zn uptake.

Original language	English
Pages (from-to)	1394-1400
Number of pages	7
Journal	Journal of Agricultural and Food Chemistry
Volume	66
Issue number	6
DOIs	https://doi.org/10.1021/acs.jafc.7b04905
Publication status	Published - 14 Feb 2018

Keywords

zinc
Zn biofortification
wheat
Zn transporters
LA:DGLA
Zn biomarker

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title = "Linoleic Acid:Dihomo-γ-Linolenic Acid Ratio Predicts the Efficacy of Zn-Biofortified Wheat in Chicken (Gallus gallus)",

abstract = "The amount of Zn absorbed from Zn-biofortified wheat material has been determined using an in vivo model of Zn absorption. The erythrocyte linoleic:dihomo -γ-linolenic acid (LA:DGLA) ratio was used as a biomarker of Zn status. Two groups of chickens (n = 15) were fed different diets: a high-Zn (46.5 μg Zn g-1) and a low-Zn wheat-based diet (32.8 μg Zn g-1). Dietary Zn intakes, body weight, serum Zn, and the erythrocyte fatty acid profile were measured, and tissues were taken for gene expression analysis. Serum Zn concentrations were greater in the high Zn group (p < 0.05). Duodenal mRNA expression of various Zn transporters demonstrated expression upregulation in the birds fed a low Zn diet (n = 15, p < 0.05). The LA:DGLA ratio was higher in the birds fed the low Zn diet (p < 0.05). The higher amount of Zn in the biofortified wheat resulted in a greater Zn uptake.",

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author = "Marija Knez and Elad Tako and Raymond Glahn and Nikolai Kolba and {de Courcy-Ireland}, Emma and James Stangoulis",

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doi = "10.1021/acs.jafc.7b04905",

language = "English",

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AU - Knez, Marija

AU - Tako, Elad

AU - Glahn, Raymond

AU - Kolba, Nikolai

AU - de Courcy-Ireland, Emma

AU - Stangoulis, James

PY - 2018/2/14

Y1 - 2018/2/14

N2 - The amount of Zn absorbed from Zn-biofortified wheat material has been determined using an in vivo model of Zn absorption. The erythrocyte linoleic:dihomo -γ-linolenic acid (LA:DGLA) ratio was used as a biomarker of Zn status. Two groups of chickens (n = 15) were fed different diets: a high-Zn (46.5 μg Zn g-1) and a low-Zn wheat-based diet (32.8 μg Zn g-1). Dietary Zn intakes, body weight, serum Zn, and the erythrocyte fatty acid profile were measured, and tissues were taken for gene expression analysis. Serum Zn concentrations were greater in the high Zn group (p < 0.05). Duodenal mRNA expression of various Zn transporters demonstrated expression upregulation in the birds fed a low Zn diet (n = 15, p < 0.05). The LA:DGLA ratio was higher in the birds fed the low Zn diet (p < 0.05). The higher amount of Zn in the biofortified wheat resulted in a greater Zn uptake.

AB - The amount of Zn absorbed from Zn-biofortified wheat material has been determined using an in vivo model of Zn absorption. The erythrocyte linoleic:dihomo -γ-linolenic acid (LA:DGLA) ratio was used as a biomarker of Zn status. Two groups of chickens (n = 15) were fed different diets: a high-Zn (46.5 μg Zn g-1) and a low-Zn wheat-based diet (32.8 μg Zn g-1). Dietary Zn intakes, body weight, serum Zn, and the erythrocyte fatty acid profile were measured, and tissues were taken for gene expression analysis. Serum Zn concentrations were greater in the high Zn group (p < 0.05). Duodenal mRNA expression of various Zn transporters demonstrated expression upregulation in the birds fed a low Zn diet (n = 15, p < 0.05). The LA:DGLA ratio was higher in the birds fed the low Zn diet (p < 0.05). The higher amount of Zn in the biofortified wheat resulted in a greater Zn uptake.

KW - zinc

KW - Zn biofortification

KW - wheat

KW - Zn transporters

KW - LA:DGLA

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DO - 10.1021/acs.jafc.7b04905

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JO - Journal of Agricultural and Food Chemistry

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SN - 0021-8561

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ER -

Linoleic Acid:Dihomo-γ-Linolenic Acid Ratio Predicts the Efficacy of Zn-Biofortified Wheat in Chicken (Gallus gallus)

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Keywords

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