Liquid-chromatographic determination of 5-fluorocytosine

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19 Citations (Scopus)


We report a 'high-performance' liquid-chromatographic method for measuring 5-fluorocytosine in plasma and cerebrospinal fluid. After deproteinization with trichloroacetic acid, the supernates are chromatographed on a reversed-phase (C18) column. Response to concentration is linear in the range of 5 to 200 mg/L, with ultraviolet detection at 276 nm. The assay requires only 0.1 mL of plasma, is reproducible, and may be performed in less than 12 min. 5-Fluorocytosine concentrations determined by this procedure correlated well with those obtained by spectrofluorometry, although the present method is more specific with no observable interference from co-administered amphotericin B and most other commonly encountered drugs, including salicylate. This method is applicable to the routine therapeutic monitoring of pediatric and adult patients as well as to pharmacokinetic studies.

Original languageEnglish
Pages (from-to)117-119
Number of pages3
JournalClinical Chemistry
Issue number1
Publication statusPublished - 1 Jan 1980
Externally publishedYes


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