Localization of catecholamine fluorescence and retrogradely transported horseradish peroxidase within the same nerve cell

W. W. Blessing, J. B. Furness, M. Costa, J. P. Chalmers

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

A technique that enables both catecholamines (CA) and horseradish peroxidase (HRP) to be localized in the same cell is described. HPR was injected into the spinal cord and three days later rabbits were perfused with a mixture of 4% formaldehyde and 0.5% glutaraldehyde to produce a fluorescence reaction in CA-containing cells. Subsequently, HRP was localized in the same neurons using tetramethylbenzidine.

Original languageEnglish
Pages (from-to)311-315
Number of pages5
JournalNeuroscience letters
Volume9
Issue number4
DOIs
Publication statusPublished - Oct 1978

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