Localization of neurotrophin-3-like immunoreactivity in peripheral tissues of the rat

Xin Fu Zhou, Robert A. Rush

    Research output: Contribution to journalArticlepeer-review

    72 Citations (Scopus)


    Neurotrophin-3 (NT-3) mRNA is widely distributed in both the peripheral and central nervous systems but neither the distribution of the native factor nor its physiological function is known. In the present study we produced and characterized an antibody to a synthetic peptide and showed that it specifically recognised endogenous rat and recombinant human NT-3 (rNT-3), but not mouse nerve growth factor and recombinant brain derived-neurotrophic factor. NT-3-like immunoreactivity (NT-3-ir) was detected within the distal tubular cells of the kidney, the zona glomerulosa and reticularis of the adrenal cortex, ganglion cells of the adrenal medulla, red pulp of the spleen, epithelial cells of the intestinal villi and parenchymal cells of the liver. Within peripheral ganglia, NT-3-ir was observed in a subpopulation of large sensory neurons of dorsal root, trigeminal and cochleovestibular ganglia but not in principle neurons of the sympathetic ganglia. These results provide the first evidence for the localization of NT-3-ir and indicate its presence in various peripheral organs and large sensory neurons. We conclude that NT-3 may function outside the nervous system in addition to a neurotrophic role within large sensory neurons.

    Original languageEnglish
    Pages (from-to)189-199
    Number of pages11
    JournalBrain Research
    Issue number2
    Publication statusPublished - 10 Sep 1993


    • Immunohistochemistry
    • Kidney
    • Liver
    • Neurotrophin
    • Neurotrophin-3
    • Rat
    • Sensory neuron


    Dive into the research topics of 'Localization of neurotrophin-3-like immunoreactivity in peripheral tissues of the rat'. Together they form a unique fingerprint.

    Cite this