TY - JOUR
T1 - Lysogeny and sporulation in Bacillus isolates from the Gulf of Mexico
AU - Mobberley, Jennifer
AU - Nathan Authement, R.
AU - Segall, Anca M.
AU - Edwards, Robert A.
AU - Slepecky, R. A.
AU - Paul, J. H.
PY - 2010/2
Y1 - 2010/2
N2 - Eleven Bacillus isolates from the surface and subsurface waters of the Gulf of Mexico were examined for their capacity to sporulate and harbor prophages. Occurrence of sporulation in each isolate was assessed through decoyinine induction, and putative lysogens were identified by prophage induction by mitomycin C treatment. No obvious correlation between ability to sporulate and prophage induction was found. Four strains that contained inducible virus-like particles (YLPs) were shown to sporulate. Four strains did not produce spores upon induction by decoyinine but contained inducible VLPs. Two of the strains did not produce virus-like particles or sporulate significantly upon induction. Isolate B14905 had a high level of virus-like particle production and a high occurrence of sporulation and was further examined by genomic sequencing in an attempt to shed light on the relationship between sporulation and lysogeny. In silico analysis of the B14905 genome revealed four prophage-like regions, one of which was independently sequenced from a mitomycin C-induced lysáte. Based on PCR and transmission electron microscopy (TEM) analysis of an Induced phage lysáte, one is a noninducible phage remnant, one may be a defective phage-like bacteriocin, and two were inducible prophages. One of the inducible phages contained four putative transcriptional regulators, one of which was a SinR-like regulator that may be involved in the regulation of host sporulation. Isolates that both possess the capacity to sporulate and contain temperate phage may be well adapted for survival in the oligotrophic ocean.
AB - Eleven Bacillus isolates from the surface and subsurface waters of the Gulf of Mexico were examined for their capacity to sporulate and harbor prophages. Occurrence of sporulation in each isolate was assessed through decoyinine induction, and putative lysogens were identified by prophage induction by mitomycin C treatment. No obvious correlation between ability to sporulate and prophage induction was found. Four strains that contained inducible virus-like particles (YLPs) were shown to sporulate. Four strains did not produce spores upon induction by decoyinine but contained inducible VLPs. Two of the strains did not produce virus-like particles or sporulate significantly upon induction. Isolate B14905 had a high level of virus-like particle production and a high occurrence of sporulation and was further examined by genomic sequencing in an attempt to shed light on the relationship between sporulation and lysogeny. In silico analysis of the B14905 genome revealed four prophage-like regions, one of which was independently sequenced from a mitomycin C-induced lysáte. Based on PCR and transmission electron microscopy (TEM) analysis of an Induced phage lysáte, one is a noninducible phage remnant, one may be a defective phage-like bacteriocin, and two were inducible prophages. One of the inducible phages contained four putative transcriptional regulators, one of which was a SinR-like regulator that may be involved in the regulation of host sporulation. Isolates that both possess the capacity to sporulate and contain temperate phage may be well adapted for survival in the oligotrophic ocean.
UR - http://www.scopus.com/inward/record.url?scp=75749138136&partnerID=8YFLogxK
U2 - 10.1128/AEM.01710-09
DO - 10.1128/AEM.01710-09
M3 - Article
C2 - 20008174
AN - SCOPUS:75749138136
SN - 0099-2240
VL - 76
SP - 829
EP - 842
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 3
ER -