TY - JOUR
T1 - Measurement of human dopamine β hydroxylase in serum by homologous radioimmunoassay
AU - Rush, R. A.
AU - Thomas, P. E.
AU - Udenfriend, S.
PY - 1975/2
Y1 - 1975/2
N2 - Human serum dopamine β hydroxylase [3,4 dihydroxyphenylethylamine, ascorbate: oxygen oxidoreductase (β hydroxylating), EC 1.14.17.1] levels were determined in 52 examples with a newly devised radioimmunoassay that utilizes purified human enzyme and its specific antiserum. The values obtained were in excellent agreement (correlation coefficient r= 0.98) with those obtained by measuring enzymatic activity in the same sera. Either assay technique, enzymatic or homologous radioimmunoassay, can be used for measurement of the enzyme in normal human serum.
AB - Human serum dopamine β hydroxylase [3,4 dihydroxyphenylethylamine, ascorbate: oxygen oxidoreductase (β hydroxylating), EC 1.14.17.1] levels were determined in 52 examples with a newly devised radioimmunoassay that utilizes purified human enzyme and its specific antiserum. The values obtained were in excellent agreement (correlation coefficient r= 0.98) with those obtained by measuring enzymatic activity in the same sera. Either assay technique, enzymatic or homologous radioimmunoassay, can be used for measurement of the enzyme in normal human serum.
UR - http://www.scopus.com/inward/record.url?scp=0016606552&partnerID=8YFLogxK
U2 - 10.1073/pnas.72.2.750
DO - 10.1073/pnas.72.2.750
M3 - Article
C2 - 1054853
AN - SCOPUS:0016606552
SN - 0027-8424
VL - 72
SP - 750
EP - 752
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 2
ER -