TY - JOUR
T1 - Mechanistic Model of Rothia mucilaginosa Adaptation toward Persistence in the CF Lung, Based on a Genome Reconstructed from Metagenomic Data
T2 - Reconstructed from Metagenomic Data
AU - Lim, Yan Wei
AU - Schmieder, Robert
AU - Haynes, Matthew
AU - Furlan, Mike
AU - Matthews, T. David
AU - Whiteson, Katrine
AU - Poole, Stephen J.
AU - Hayes, Christopher S.
AU - Low, David A.
AU - Maughan, Heather
AU - Edwards, Robert
AU - Conrad, Douglas
AU - Rohwer, Forest
N1 - This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. PLOS applies the Creative Commons Attribution (CC BY) license to articles and other works we publish.
PY - 2013/5/30
Y1 - 2013/5/30
N2 - The impaired mucociliary clearance in individuals with Cystic Fibrosis (CF) enables opportunistic pathogens to colonize CF lungs. Here we show that Rothia mucilaginosa is a common CF opportunist that was present in 83% of our patient cohort, almost as prevalent as Pseudomonas aeruginosa (89%). Sequencing of lung microbial metagenomes identified unique R. mucilaginosa strains in each patient, presumably due to evolution within the lung. The de novo assembly of a near-complete R. mucilaginosa (CF1E) genome illuminated a number of potential physiological adaptations to the CF lung, including antibiotic resistance, utilization of extracellular lactate, and modification of the type I restriction-modification system. Metabolic characteristics predicted from the metagenomes suggested R. mucilaginosa have adapted to live within the microaerophilic surface of the mucus layer in CF lungs. The results also highlight the remarkable evolutionary and ecological similarities of many CF pathogens; further examination of these similarities has the potential to guide patient care and treatment.
AB - The impaired mucociliary clearance in individuals with Cystic Fibrosis (CF) enables opportunistic pathogens to colonize CF lungs. Here we show that Rothia mucilaginosa is a common CF opportunist that was present in 83% of our patient cohort, almost as prevalent as Pseudomonas aeruginosa (89%). Sequencing of lung microbial metagenomes identified unique R. mucilaginosa strains in each patient, presumably due to evolution within the lung. The de novo assembly of a near-complete R. mucilaginosa (CF1E) genome illuminated a number of potential physiological adaptations to the CF lung, including antibiotic resistance, utilization of extracellular lactate, and modification of the type I restriction-modification system. Metabolic characteristics predicted from the metagenomes suggested R. mucilaginosa have adapted to live within the microaerophilic surface of the mucus layer in CF lungs. The results also highlight the remarkable evolutionary and ecological similarities of many CF pathogens; further examination of these similarities has the potential to guide patient care and treatment.
UR - http://www.scopus.com/inward/record.url?scp=84878493219&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0064285
DO - 10.1371/journal.pone.0064285
M3 - Article
C2 - 23737977
AN - SCOPUS:84878493219
SN - 1932-6203
VL - 8
JO - PLoS One
JF - PLoS One
IS - 5
M1 - e64285
ER -