Mesenchymal stem cell attachment to peptide density gradients on porous silicon generated by electrografting

Lauren Clements, Peng-Yuan Wang, Frances Harding, Wei-Bor Tsai, Helmut Thissen, Nicolas Voelcker

    Research output: Contribution to journalArticle

    27 Citations (Scopus)

    Abstract

    Chemical gradients of ethyl-6-bromohexanoate (EBH) were generated over porous silicon (pSi) substrates via electrochemical attachment. Immobilised ester moieties were hydrolysed and activated to produce a gradient of functional carboxylic acid groups. After subsequent immobilisation of cyclic RGD (cRGD), the surfaces were used to screen the extent of rat mesenchymal stem cell (MSC) attachment. Mapping of surface chemistry was carried out by means of infrared microscopy and X-ray photoelectron spectroscopy (XPS). MSC culture studies showed that short-term cell attachment responded to the cRGD density present on the gradient surface.

    Original languageEnglish
    Pages (from-to)1440-1445
    Number of pages6
    JournalPhysica Status Solidi A-Applications and Materials Science
    Volume208
    Issue number6
    DOIs
    Publication statusPublished - Jun 2011

    Keywords

    • biomaterial
    • electrochemistry
    • gradient surfaces
    • porous silicon
    • surface chemistry

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