Mesenchymal stem cell attachment to peptide density gradients on porous silicon generated by electrografting

Lauren Clements; Peng-Yuan Wang; Frances Harding; Wei-Bor Tsai; Helmut Thissen; Nicolas Voelcker

doi:10.1002/pssa.201000320

Mesenchymal stem cell attachment to peptide density gradients on porous silicon generated by electrografting

Lauren Clements, Peng-Yuan Wang, Frances Harding, Wei-Bor Tsai, Helmut Thissen, Nicolas Voelcker

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Chemical gradients of ethyl-6-bromohexanoate (EBH) were generated over porous silicon (pSi) substrates via electrochemical attachment. Immobilised ester moieties were hydrolysed and activated to produce a gradient of functional carboxylic acid groups. After subsequent immobilisation of cyclic RGD (cRGD), the surfaces were used to screen the extent of rat mesenchymal stem cell (MSC) attachment. Mapping of surface chemistry was carried out by means of infrared microscopy and X-ray photoelectron spectroscopy (XPS). MSC culture studies showed that short-term cell attachment responded to the cRGD density present on the gradient surface.

Original language	English
Pages (from-to)	1440-1445
Number of pages	6
Journal	Physica Status Solidi A-Applications and Materials Science
Volume	208
Issue number	6
DOIs	https://doi.org/10.1002/pssa.201000320
Publication status	Published - Jun 2011

Keywords

biomaterial
electrochemistry
gradient surfaces
porous silicon
surface chemistry

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10.1002/pssa.201000320

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abstract = "Chemical gradients of ethyl-6-bromohexanoate (EBH) were generated over porous silicon (pSi) substrates via electrochemical attachment. Immobilised ester moieties were hydrolysed and activated to produce a gradient of functional carboxylic acid groups. After subsequent immobilisation of cyclic RGD (cRGD), the surfaces were used to screen the extent of rat mesenchymal stem cell (MSC) attachment. Mapping of surface chemistry was carried out by means of infrared microscopy and X-ray photoelectron spectroscopy (XPS). MSC culture studies showed that short-term cell attachment responded to the cRGD density present on the gradient surface.",

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AU - Tsai, Wei-Bor

AU - Thissen, Helmut

AU - Voelcker, Nicolas

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AB - Chemical gradients of ethyl-6-bromohexanoate (EBH) were generated over porous silicon (pSi) substrates via electrochemical attachment. Immobilised ester moieties were hydrolysed and activated to produce a gradient of functional carboxylic acid groups. After subsequent immobilisation of cyclic RGD (cRGD), the surfaces were used to screen the extent of rat mesenchymal stem cell (MSC) attachment. Mapping of surface chemistry was carried out by means of infrared microscopy and X-ray photoelectron spectroscopy (XPS). MSC culture studies showed that short-term cell attachment responded to the cRGD density present on the gradient surface.

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KW - electrochemistry

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KW - surface chemistry

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Mesenchymal stem cell attachment to peptide density gradients on porous silicon generated by electrografting

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Keywords

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