Metabolic activity of Glomus intraradices in Arum- and Paris-type arbuscular mycorrhizal colonization

Ingrid M. van Aarle; Timothy R. Cavagnaro; Sally E. Smith; F. Andrew Smith; Sandy Dickson

doi:10.1111/j.1469-8137.2005.01340.x

Metabolic activity of Glomus intraradices in Arum- and Paris-type arbuscular mycorrhizal colonization

Ingrid M. van Aarle, Timothy R. Cavagnaro, Sally E. Smith, F. Andrew Smith, Sandy Dickson

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

• Colonization of two plant species by Glomus intraradices was studied to investigate the two morphological types (Arum and Paris), their symbiotic interfaces and metabolic activities.

• Root pieces and sections were stained to observe the colonization and metabolic activity of all mycorrhizal structures.

• There were no growth responses observed in the plants caused by mycorrhizal symbiosis. The two morphological types had a similar percentage of root colonized, but the Arum-type had higher metabolic activity. Most of the mycorrhizal structures (88%) showed succinate dehydrogenase activity; about half showed acid phosphatase activity; and a small percentage showed alkaline phosphatase activity. Phosphatase activity was highest in arbuscules and low in intercellular hyphae in the Arum-type colonization. In the Paris-type, hyphal coils and arbusculate coils showed a similar intermediate percentage of phosphatase activity.

• We conclude that acid phosphatase is more important than alkaline phosphatase in both colonization types. We discuss the possibility that, whereas arbuscules in Arum-type are the main site for phosphorus release to the host plant, both the hyphal and arbusculate coils may be involved in the Paris-type.

Original language	English
Pages (from-to)	611-618
Number of pages	8
Journal	NEW PHYTOLOGIST
Volume	166
Issue number	2
DOIs	https://doi.org/10.1111/j.1469-8137.2005.01340.x
Publication status	Published - May 2005
Externally published	Yes

Keywords

Arbuscular mycorrhiza
Arbuscule
Coil
ELF (enzyme-labelled fluorescence)
LSCM (laser scanning confocal microscope)
Metabolic activity
Phosphatase
Succinate dehydrogenase

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title = "Metabolic activity of Glomus intraradices in Arum- and Paris-type arbuscular mycorrhizal colonization",

abstract = "• Colonization of two plant species by Glomus intraradices was studied to investigate the two morphological types (Arum and Paris), their symbiotic interfaces and metabolic activities. • Root pieces and sections were stained to observe the colonization and metabolic activity of all mycorrhizal structures. • There were no growth responses observed in the plants caused by mycorrhizal symbiosis. The two morphological types had a similar percentage of root colonized, but the Arum-type had higher metabolic activity. Most of the mycorrhizal structures (88%) showed succinate dehydrogenase activity; about half showed acid phosphatase activity; and a small percentage showed alkaline phosphatase activity. Phosphatase activity was highest in arbuscules and low in intercellular hyphae in the Arum-type colonization. In the Paris-type, hyphal coils and arbusculate coils showed a similar intermediate percentage of phosphatase activity. • We conclude that acid phosphatase is more important than alkaline phosphatase in both colonization types. We discuss the possibility that, whereas arbuscules in Arum-type are the main site for phosphorus release to the host plant, both the hyphal and arbusculate coils may be involved in the Paris-type.",

keywords = "Arbuscular mycorrhiza, Arbuscule, Coil, ELF (enzyme-labelled fluorescence), LSCM (laser scanning confocal microscope), Metabolic activity, Phosphatase, Succinate dehydrogenase",

author = "{van Aarle}, {Ingrid M.} and Cavagnaro, {Timothy R.} and Smith, {Sally E.} and Smith, {F. Andrew} and Sandy Dickson",

year = "2005",

month = may,

doi = "10.1111/j.1469-8137.2005.01340.x",

language = "English",

volume = "166",

pages = "611--618",

journal = "NEW PHYTOLOGIST",

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T1 - Metabolic activity of Glomus intraradices in Arum- and Paris-type arbuscular mycorrhizal colonization

AU - van Aarle, Ingrid M.

AU - Cavagnaro, Timothy R.

AU - Smith, Sally E.

AU - Smith, F. Andrew

AU - Dickson, Sandy

PY - 2005/5

Y1 - 2005/5

N2 - • Colonization of two plant species by Glomus intraradices was studied to investigate the two morphological types (Arum and Paris), their symbiotic interfaces and metabolic activities. • Root pieces and sections were stained to observe the colonization and metabolic activity of all mycorrhizal structures. • There were no growth responses observed in the plants caused by mycorrhizal symbiosis. The two morphological types had a similar percentage of root colonized, but the Arum-type had higher metabolic activity. Most of the mycorrhizal structures (88%) showed succinate dehydrogenase activity; about half showed acid phosphatase activity; and a small percentage showed alkaline phosphatase activity. Phosphatase activity was highest in arbuscules and low in intercellular hyphae in the Arum-type colonization. In the Paris-type, hyphal coils and arbusculate coils showed a similar intermediate percentage of phosphatase activity. • We conclude that acid phosphatase is more important than alkaline phosphatase in both colonization types. We discuss the possibility that, whereas arbuscules in Arum-type are the main site for phosphorus release to the host plant, both the hyphal and arbusculate coils may be involved in the Paris-type.

AB - • Colonization of two plant species by Glomus intraradices was studied to investigate the two morphological types (Arum and Paris), their symbiotic interfaces and metabolic activities. • Root pieces and sections were stained to observe the colonization and metabolic activity of all mycorrhizal structures. • There were no growth responses observed in the plants caused by mycorrhizal symbiosis. The two morphological types had a similar percentage of root colonized, but the Arum-type had higher metabolic activity. Most of the mycorrhizal structures (88%) showed succinate dehydrogenase activity; about half showed acid phosphatase activity; and a small percentage showed alkaline phosphatase activity. Phosphatase activity was highest in arbuscules and low in intercellular hyphae in the Arum-type colonization. In the Paris-type, hyphal coils and arbusculate coils showed a similar intermediate percentage of phosphatase activity. • We conclude that acid phosphatase is more important than alkaline phosphatase in both colonization types. We discuss the possibility that, whereas arbuscules in Arum-type are the main site for phosphorus release to the host plant, both the hyphal and arbusculate coils may be involved in the Paris-type.

KW - Arbuscular mycorrhiza

KW - Arbuscule

KW - Coil

KW - ELF (enzyme-labelled fluorescence)

KW - LSCM (laser scanning confocal microscope)

KW - Metabolic activity

KW - Phosphatase

KW - Succinate dehydrogenase

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Metabolic activity of Glomus intraradices in Arum- and Paris-type arbuscular mycorrhizal colonization

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Keywords

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