Metabolism of 2-acetylaminofluorene in cultured human lymphocytes

M. E. McManus, K. J. Trainor, A. A. Morley, W. Burgess, I. Stupans, D. J. Birkett

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The capacity of lymphocytes from 23 human subjects to metabolize the model carcinogen 2-acetylaminofluorene (AAF) was assessed. These cells metabolized AAF to its 1-, 7- and N-hydroxylated metabolites. Alpha-naphthoflavone and metyrapone exhibited IC50's for all pathways of 0.02 μM and 1 mM, respectively. These data indicate that the same form of cytochrome P450 or forms with similar IC50's are mediating these reactions. No significant difference was observed between lymphocytes from smokers versus non-smokers in the N-hydroxylation of AAF, the initial step in the metabolic activation of this carcinogen. However, lymphocytes from smokers were faster detoxifiers of AAF as seen in their increased capacity to 1- and 7-hydroxylate AAF compared to lymphocytes from non-smokers.

Original languageEnglish
Pages (from-to)409-418
Number of pages10
JournalResearch Communications in Chemical Pathology and Pharmacology
Volume55
Issue number3
Publication statusPublished - 1987

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