Methods for study of mutations and mutagenesis in human lymphocytes

A. A. Morley; K. J. Trainor; J. L. Dempsey; R. S. Seshadri

doi:10.1016/0165-1161(85)90005-6

Methods for study of mutations and mutagenesis in human lymphocytes

A. A. Morley, K. J. Trainor, J. L. Dempsey, R. S. Seshadri

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Abstract

Detailed methods are presented for measurement and study of in vivo mutations and in vitro mutagenesis in human lymphocytes. The methods described include preparation of conditioned medium containing inteleukin-2, enumeration of mutant clones, in vitro mutagenesis, and expansion of mutant clones for further study.

Original language	English
Pages (from-to)	363-367
Number of pages	5
Journal	Mutation Research/Environmental Mutagenesis and Related Subjects
Volume	147
Issue number	6
DOIs	https://doi.org/10.1016/0165-1161(85)90005-6
Publication status	Published - Dec 1985

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@article{d8c0082ef3374f4a842df665cbb301bb,

title = "Methods for study of mutations and mutagenesis in human lymphocytes",

abstract = "Detailed methods are presented for measurement and study of in vivo mutations and in vitro mutagenesis in human lymphocytes. The methods described include preparation of conditioned medium containing inteleukin-2, enumeration of mutant clones, in vitro mutagenesis, and expansion of mutant clones for further study.",

author = "Morley, {A. A.} and Trainor, {K. J.} and Dempsey, {J. L.} and Seshadri, {R. S.}",

year = "1985",

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T1 - Methods for study of mutations and mutagenesis in human lymphocytes

AU - Morley, A. A.

AU - Trainor, K. J.

AU - Dempsey, J. L.

AU - Seshadri, R. S.

PY - 1985/12

Y1 - 1985/12

N2 - Detailed methods are presented for measurement and study of in vivo mutations and in vitro mutagenesis in human lymphocytes. The methods described include preparation of conditioned medium containing inteleukin-2, enumeration of mutant clones, in vitro mutagenesis, and expansion of mutant clones for further study.

AB - Detailed methods are presented for measurement and study of in vivo mutations and in vitro mutagenesis in human lymphocytes. The methods described include preparation of conditioned medium containing inteleukin-2, enumeration of mutant clones, in vitro mutagenesis, and expansion of mutant clones for further study.

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U2 - 10.1016/0165-1161(85)90005-6

DO - 10.1016/0165-1161(85)90005-6

M3 - Article

C2 - 4058450

AN - SCOPUS:0022392892

SN - 0165-1161

VL - 147

SP - 363

EP - 367

JO - Mutation Research/Environmental Mutagenesis and Related Subjects

JF - Mutation Research/Environmental Mutagenesis and Related Subjects

IS - 6

ER -

Methods for study of mutations and mutagenesis in human lymphocytes

Abstract

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