Metoclopramide improves splenocyte immune function and restores plasma corticosteroid levels after hemorrhagic shock

R. Zellweger, M. W. Wichmann, A. Avala, C. M. Demaso, I. H. Chaudry

Research output: Contribution to journalArticlepeer-review

Abstract

To study whether metoclopramide {MCP) produces any beneficial effects on the depressed splenocyte (SPL) immune function following severe hemorrhage, we randomized male C3H/HeN mice into one of 3 groups (group 1: SHAM; group 2: hemorrhage (HEM) ; group 3: hemorrhage+MCP (HEM+MCP). Hemorrhaged animals were bled to and maintained at a mean arterial blood pressure of 35 ±BmmHg for 60mins. The shed blood was then returned along with lactated Ringer's (2× the shed blood volume). Group 2 received saline-vehicle; group 3 was treated with MCP (100μg/ 100g BW, S.C.). Two hours after the experiment, the animals were sacrificed and splenocyte (SPL) cultures established and plasma corticosterone levels were determined by RIA. The results (mean ± SEM) were SPL Proliferation IL-2 IL-3 Corticosterone (CPM) (Uml) (U/ml) (ng/ml) SHAM 265950 ± 25131 17.5 ± 2.5 175 ± 18 289 ± 22.8 HEM 131250 ± 7528 10.4 ± 1.2 87 ±11 393 ± 16.1 HEM+MCP 259020 ± 12969 18.6 ± 1.2 152 ± 10.7 266 ± 24.7 N=6/group, ANOVA, Tukey's Test, p<0.05 vs. shams The depressed splenocyte proliferative capacity, as well as their ability to release IL-2 and IL-3, were markedly improved in hemorrhaged animals which were treated with MCP. Furthermore, treatment with MCP normalized the elevated blood corticosterone levels seen after severe hemorrhage. These results support the concept that the immunosuppression following hemorrhage may be mediated by hormones from the hypothalamic-pituitary-adrenal axis.

Original languageEnglish
Pages (from-to)A597
JournalFASEB Journal
Volume10
Issue number3
Publication statusPublished - 1996
Externally publishedYes

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