MHC in a monogamous lizard - characterization of Class I MHC genes in the Australian skink (Tiliqua rugosa)

Talat Mina Hojat Ansari Komachali, Terry Bertozzi, Robert Miller, Michael Gardner

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    The major histocompatibility complex (MHC) is a highly variable region of vertebrate genomes that encodes cellular proteins involved in the immune response. In addition to the benefits of MHC research in understanding the genetic basis of host resistance to disease, the MHC is an ideal candidate for studying genetic diversity under strong natural selection. However, the MHC of many non-model vertebrate taxa are poorly characterized, hindering an understanding of disease resistance and its application to conservation genetics in these groups. Squamates (lizards and snakes) remain particularly underrepresented despite their being the most diverse order of non-avian sauropsids. We characterized MHC class I sequence diversity from an Australian skink, the sleepy lizard (. Tiliqua rugosa), using both cDNA and genomic sequence data and also present genomic class I sequences from the related skinks Tiliqua adelaidensis and Egernia stokesii. Phylogenetic analysis of Tiliqua and other published sqamate MHC class I sequences suggest that MHC diverged very early in Tiliqua compared with the other studied squamates. We identified at least 4 classical MHC class I loci in T. rugosa and also shared polymorphism among T. rugosa, T. adelaidensis and E. stokesii in the sequences encoding peptide-binding α1 and α2 domains.

    Original languageEnglish
    Pages (from-to)320-327
    Number of pages8
    JournalDevelopmental and Comparative Immunology
    Volume53
    Issue number2
    DOIs
    Publication statusPublished - 1 Dec 2015

    Keywords

    • CDNA
    • Egernia stokesii
    • Genomic DNA
    • MHC
    • Scincadae
    • Squamates
    • Tiliqua adelaidensis
    • Tiliqua rugosa

    Fingerprint Dive into the research topics of 'MHC in a monogamous lizard - characterization of Class I MHC genes in the Australian skink (Tiliqua rugosa)'. Together they form a unique fingerprint.

    Cite this