Micellar electrokinetic capillary chromatographic determination of pirfenidone and 5-carboxy-pirfenidone by direct injection of plasma from patients receiving treatment for idiopathic pulmonary fibrosis (IPF)

Salvatore Sotgia, Alessandro G. Fois, Elisabetta Sotgiu, Angelo Zinellu, Panagiotis Paliogiannis, Arduino A. Mangoni, Ciriaco Carru

Research output: Contribution to journalArticlepeer-review

Abstract

A micellar electrokinetic capillary chromatographic method, a variant of capillary electrophoresis, was developed for the detection and measurement of pirfenidone and 5-carboxy-pirfenidone by direct injection of plasma from patients under treatment for idiopathic pulmonary fibrosis. To achieve the best separation a 35 mmol/L N-lauroylsarcosine sodium salt surfactant added with a 16 mmol/L sodium 1-heptanesulfonate solution was used as a running buffer. Analytes were eluted in a fairly short time, 7.35 and 9.40 min for pirfenidone and 5-carboxy-pirfenidone, respectively, at 15 °C and with an applied voltage of 30 kV in an 80 cm, 75 µm i.d. uncoated fused-silica capillary. In the range of 6.25–200 µmol/L, calibration curves exhibited acceptable linearity with the coefficient of determination higher than 0.999. The recovery of analytes spiked in samples of plasma was found to be 95–109% and 93–106% for pirfenidone and 5-carboxy-pirfenidone, respectively. Intra- and inter-day precisions were around 5%. LOD and LOQ for pirfenidone and 5-carboxy-pirfenidone were, respectively, 1.0 and 4.0 µmol/L. The method, applied to measure analytes in six idiopathic pulmonary fibrosis male patients, provided mean concentrations of 68.91 ± 26.31 and 36.21 ± 26.39 µmol/L for pirfenidone and 5-carboxy-pirfenidone, respectively.

Original languageEnglish
Article number104536
JournalMICROCHEMICAL JOURNAL
Volume154
DOIs
Publication statusPublished - May 2020

Keywords

  • Capillary electrophoresis
  • Drug therapy monitoring
  • Micellar electrokinetic capillary chromatography

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