Micro-flow photosynthesis of new dienophiles for inverse-electron-demand Diels–Alder reactions.Potential applications for pretargeted in vivo PETimaging

Emilie Billaud, Elnaz Shahbazali, Muneer Ahamed, Frederik Cleeren, Timothy Noel, Michel Koole, Alfons Verbruggen, Volker Hessel, Guy Bormans

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    23 Citations (Scopus)

    Abstract

    Pretargeted PET imaging has emerged as an effective two-step in vivo approach that combines the superior affinity and selectivity of antibodies with the rapid pharmacokinetics and favorable dosimetry of smaller molecules radiolabeled with short-lived radionuclides. This approach can be based on the bioorthogonal inverse-electron-demand Diels-Alder (IEDDA) reaction between tetrazines and trans-cyclooctene (TCO) derivatives. We aimed to develop new [18F]TCO-dienophiles with high reactivity for IEDDA reactions, and favorable in vivo stability and pharmacokinetics. New dienophiles were synthesized using an innovative micro-flow photochemistry process, and their reaction kinetics with a tetrazine were determined. In vivo stability and biodistribution of the most promising 18F-radiolabeled-TCO-derivative ([18F]3) was investigated, and its potential for in vivo pretargeted PET imaging was assessed in tumor-bearing mice. We demonstrated that [18F]3 is a suitable dienophile for IEDDA reactions and for pretargeting applications.

    Original languageEnglish
    Pages (from-to)1251-1258
    Number of pages8
    JournalChemical Science
    Volume8
    Issue number2
    DOIs
    Publication statusPublished - 2017

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    Billaud, E., Shahbazali, E., Ahamed, M., Cleeren, F., Noel, T., Koole, M., Verbruggen, A., Hessel, V., & Bormans, G. (2017). Micro-flow photosynthesis of new dienophiles for inverse-electron-demand Diels–Alder reactions.Potential applications for pretargeted in vivo PETimaging. Chemical Science, 8(2), 1251-1258. https://doi.org/10.1039/c6sc02933g