Microbial cell lysis and nucleic acid extraction via nanofluidic channel

Krishna Kant; Jeongha Yoo; Steven Amos; Mason Erkelens; Craig Priest; Joseph Shapter; Dusan Losic

doi:10.1039/c5ra01336d

Microbial cell lysis and nucleic acid extraction via nanofluidic channel

Krishna Kant, Jeongha Yoo, Steven Amos, Mason Erkelens, Craig Priest, Joseph Shapter, Dusan Losic

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

This paper presents a microfluidic device with a nano-channel prepared by focused ion beam (FIB) milling for microbial cell lysis and nucleic acid extraction. The device prepared in a quartz slide combines two microchannels connected with one nanochannel in the middle with dimensions of 100 nm (width) and 250 nm (depth) and 1 μm (length). The capturing of a single or a few cells and their lysis for extraction of DNA in nanochannel were demonstrated. The purity and quantity of the extracted genetic material was tested using a UV spectrometer. The results revealed that the extraction methodology was successful and may be well-suited to integration in analytical lab-on-a-chip devices. This journal is

Original language	English
Pages (from-to)	23886-23891
Number of pages	6
Journal	RSC Advances
Volume	5
Issue number	30
DOIs	https://doi.org/10.1039/c5ra01336d
Publication status	Published - 2015

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AU - Kant, Krishna

AU - Yoo, Jeongha

AU - Amos, Steven

AU - Erkelens, Mason

AU - Priest, Craig

AU - Shapter, Joseph

AU - Losic, Dusan

PY - 2015

Y1 - 2015

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AB - This paper presents a microfluidic device with a nano-channel prepared by focused ion beam (FIB) milling for microbial cell lysis and nucleic acid extraction. The device prepared in a quartz slide combines two microchannels connected with one nanochannel in the middle with dimensions of 100 nm (width) and 250 nm (depth) and 1 μm (length). The capturing of a single or a few cells and their lysis for extraction of DNA in nanochannel were demonstrated. The purity and quantity of the extracted genetic material was tested using a UV spectrometer. The results revealed that the extraction methodology was successful and may be well-suited to integration in analytical lab-on-a-chip devices. This journal is

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JO - RSC Advances

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Microbial cell lysis and nucleic acid extraction via nanofluidic channel

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