Microbispora Dominate Diversity of Endophytic Actinobacteria from Australian Rice Plants

Fitri Widiantini, Christopher Franco

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A study to detect the diversity of endophytic Actinobacteria from Australian rice was conducted using culture-dependent and culture-independent methods. Rice samples were collected from the rice growing area near Yanco, New South Wales, Australia. Isolation of the endophytic Actinobacteria was done over two consecutive growing seasons. The results demonstrated that most isolates were obtained from plants 10 weeks and older, and only a few were found in younger plants. Microbispora spp. were the most commonly isolated endophytic Actinobacteria (94%) with Streptomyces spp. and other genera present at lower numbers (6%). The culture-dependent method findings were confirmed by T-RFLP profile analysis. Restriction digests using HhaI and RsaI also showed an abundance of terminal restriction fragments (TRFs) profiles related to the genus Microbispora. Furthermore, other biological properties of the endophytic Actinobacteria isolates were also determined. Four isolates, Saccharothrix OSH21, Saccharopolyspora OSR26, Streptomyces OSR46 and Microbispora OSR61, were found to suppress the growth of the pathogenic fungus Rhizoctonia solani. Moreover, these isolates might be able to promote plant growth by producing indole acetic acid or to solubilise phosphate making this nutrient available for plant uptake.
Original languageEnglish
Title of host publicationEndophytes for a Growing World
Subtitle of host publicationPart III - Diversity and Community Ecology of Endophytes
EditorsTrevor R. Hodkinson, Fiona M. Doohan, Matthew J. Saunders, Brian R. Murphy
Place of PublicationUnited Kingdom
PublisherCambridge University Press
Chapter8
Pages167-187
ISBN (Electronic)9781108607667
ISBN (Print)9781108458405
DOIs
Publication statusPublished - 2019

Keywords

  • plant sciences
  • endophytic Actinobacteria
  • Australian rice
  • Rice samples

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    Widiantini, F., & Franco, C. (2019). Microbispora Dominate Diversity of Endophytic Actinobacteria from Australian Rice Plants. In T. R. Hodkinson, F. M. Doohan, M. J. Saunders, & B. R. Murphy (Eds.), Endophytes for a Growing World: Part III - Diversity and Community Ecology of Endophytes (pp. 167-187). Cambridge University Press. https://doi.org/10.1017/9781108607667.009