TY - JOUR
T1 - Microfluidic formation of core-shell alginate microparticles for protein encapsulation and controlled release
AU - Yu, Lei
AU - Sun, Qi
AU - Hui, Yue
AU - Seth, Arjun
AU - Petrovsky, Nikolai
AU - Zhao, Chun Xia
PY - 2019/3/15
Y1 - 2019/3/15
N2 - Alginate hydrogel particles are promising delivery systems for protein encapsulation and controlled release because of their excellent biocompatibility, biodegradability, and mild gelation process. In this study, a facile microfluidic approach is developed for making uniform core-shell hydrogel microparticles. To address the challenge of protein retention within the alginate gel matrix, poly(ethyleneimine) (PEI)- and chitosan-coated alginate microparticles were fabricated demonstrating improved protein retention as well as controlled release. Furthermore, a model protein ovalbumin was loaded along with delta inulin microparticulate adjuvant into the water-core of the alginate microparticles. Compared to those microparticles with only antigen loaded, the antigen + adjuvant loaded microparticles showed a delayed and sustained release of antigen. This microfluidic approach provides a convenient method for making well-controlled alginate microgel particles with uniform size and controlled properties, and demonstrates the ability to tune the release profiles of proteins by engineering microparticle structure and properties.
AB - Alginate hydrogel particles are promising delivery systems for protein encapsulation and controlled release because of their excellent biocompatibility, biodegradability, and mild gelation process. In this study, a facile microfluidic approach is developed for making uniform core-shell hydrogel microparticles. To address the challenge of protein retention within the alginate gel matrix, poly(ethyleneimine) (PEI)- and chitosan-coated alginate microparticles were fabricated demonstrating improved protein retention as well as controlled release. Furthermore, a model protein ovalbumin was loaded along with delta inulin microparticulate adjuvant into the water-core of the alginate microparticles. Compared to those microparticles with only antigen loaded, the antigen + adjuvant loaded microparticles showed a delayed and sustained release of antigen. This microfluidic approach provides a convenient method for making well-controlled alginate microgel particles with uniform size and controlled properties, and demonstrates the ability to tune the release profiles of proteins by engineering microparticle structure and properties.
KW - Alginate microparticles
KW - Chitosan
KW - Controlled release
KW - Microfluidics
KW - Protein encapsulation
UR - http://www.scopus.com/inward/record.url?scp=85059317196&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/ARC/LP140100424
UR - http://purl.org/au-research/grants/ARC/FT140100726
U2 - 10.1016/j.jcis.2018.12.075
DO - 10.1016/j.jcis.2018.12.075
M3 - Article
C2 - 30611045
AN - SCOPUS:85059317196
SN - 0021-9797
VL - 539
SP - 497
EP - 503
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
ER -