Microfluidic PCR combined with pyrosequencing for identification of allelic variants with phenotypic associations among targeted Salmonella genes

Min Yue, Robert Schmieder, Robert A. Edwards, Shelley C. Rankin, Dieter M. Schifferli

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

A novel targeted massive parallel sequencing approach identified genetic variation in eight known or predicted fimbrial adhesins for 46 Salmonella strains. The results highlight associations between specific adhesin alleles, host species, and antimicrobial resistance. The differentiation of allelic variants has potential applications for diagnostic microbiology and epidemiological investigations.

Original languageEnglish
Pages (from-to)7480-7482
Number of pages3
JournalApplied and Environmental Microbiology
Volume78
Issue number20
DOIs
Publication statusPublished - Oct 2012
Externally publishedYes

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