Micromonospora terminaliae sp. nov., an endophytic actinobacterium isolated from the surface-sterilized stem of the medicinal plant Terminalia mucronata

Anéndophytic actinobacterium, strain TMS7^T, was isolated from the stem of a Thai medicinal plant collected from the grounds of the Phujong-Nayoa National park, Ubon Ratchathani province, Thailand. As a result of a polyphasic taxonomy study, this strain was identified as a member of the genus Micromonospora. This strain was a Gram-stain-positive, aerobic actinobacterium with well-developed substrate mycelium with hyphae forming a single microspore was non-motile. Stran TMS7^T was identified according to its 16S rRNA gene sequence as a new member of the genus Micromonospora. The closest phylogenetic members sharing a similarity were Micromonospora chersina DSM 44151^T at 99.4% and Micromonospora rosaria DSM 803^T, Micromonospora tulbaghiae TVU1^T, Micromonospora inositola DSM 43819^T and Micromonosporaéndolithica DSM 44398^T all at 99.2%. Chemotaxonomic data including cell wall components, major menaquinones and major fatty acids confirmed the affiliation of strain TMS7^T to the genus Micromonospora. The results of the phylogenetic analysis, addition to physiological and biochemical studies in combination with DNA–DNA hybridization, allowed the genotypic and phenotypic differentiation of strain TMS7^T and the most closely related species with validly published names. The name proposed for the novel species is Micromonospora terminaliae sp. nov. The type strain is TMS7^T (=DSM 101760^T=NRRL B-65345^T).